Anti-A and Anti-B titer in O group platelet

[Eagle Eye]

What is your policy on giving group platelet(SDP) to non group O recipient?

DO you determine anti-A & anti-B titer for all your O SDPs?

WHat is your SOP on performing Anti-A and anti-B titer? RT? or IAT?

What is your critical titer where you decide not to use that O SDP to non group SDPs?

Thank you for your help. I know I can count on my friends who are willing to share their knowledge.

[Eagle Eye]

What is your policy on issuing group O platelet to nongroup O patient?

[Cliff]

We perform a screen on all of our group O platelets. It's a simple one point titer of 200. If it's > 200, then we don't issue it to a non-group O.

[Eagle Eye]

Is the titer RT(15min?), IS or IAT?

[Cliff]

We do a 1:200 dilution at AHG only. It's not a titer, but more of a Pos / Neg result.

[donellda]

We try to give ABO compatible when possible. We do not have the capability of volume reduction at our facility so that is not a possibility.

[John C. Staley]

We try to limit incompatible plasma in adults to 600 ml in a 24 hour period not to exceed 1000 ml in 7 days. Not sure where this came from. Think it was the corporate transfusion medical director.

[sandy]

We limit ABO incompatible plt txs to no more than 5 units per pool.

[Geriann]

None of the above policies...we avoid giving Group O pheresis to a non O recipient when at all possible...there are times when a Group O pheresis is the only type available...then it becomes the transfusing physician's call...usually this occurs in an emergent situation in the OR.

[javvcr]

we try to use O plts in O pacient, if we can´t we wash the plts with saline. Use in 6 hrs, no more.

[csjuarez]

We try as much as possible to give ABO compatible platelets, but this is often not possible since our demand far exceeds our supply (and we have to collect all that we transfuse!). For neonates, ABO compatible is required -- if it's not available we give volume reduced. If an apheresis unit is known to be high-titer, we label it to be used only for group O patients, but don't have a policy or practice of doing titers routinely.1

[leslieweber]

Hi

We titer for anti-A only, and use 1:100 as a cutoff.

[jgatti]

We perform an anti-A and anti-B titer using a 1:100 dilution on all O apheresis platelets. We incubate at RT for 15 minutes. If the result is negative, we use the unit for non-O adult patients.

[sgoertzen]

Just wondering... from all of your experiences... what percentage of group O plateletpheresis have an Anti-A titer of less than 1:200? How about the anticipated titer values of Anti-B in a group A or O plateletpheresis?

I'm at a pediatric hospital and we spend a lot of time volume reducing platelets when we do not have plasma compatible available - which is often for group B and AB patients. We are using >45 kilos as the limit for which we no longer have to volume reduce incompatible platelets, but I am wondering whether maybe titering the product would be a better (maybe even safer) way to go. Even though we use the methods suggested by AABB, I always wonder about the final quality of the platelets after we take them through the volume reduction process.

Please e-mail me if you have some experience with this.

Thanks! Sheri Goertzen

sgoertzen@childrenscentralcal.org