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Thaw and re-freeze?


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Does anyone have experience with thawing, aliquoting and then re-freezing HPC's? We normally cryopreserve as soon as we get product and usually have it aliquoted before we get our yield results. In this case we had a great yield and it was stored in just two bags. The recipient got one and went into remission for almost 5 years. She is now relapsing and is up for a second transplant. There are enough cells in the remaining bag for another two transplants. So our director wants to know if there's any information on thawing, aliquoting and re-freezing product. Any data you have will be appreciated.

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Good idea Kate. Unfortunately, we recently did a major purge of products collected from patients who are now deceased. We had added a clause to the HPC consent that allows us to discard unused product from deceased patients. We may have a few products left from collections done before the change was made to the consent. However, the program director is still waiting to hear back from next-of-kin to see if we can discard those products, so I don't think we could use those just yet.

Our hematology lab does the viability studies but their results have been all over the place. I will bring it up to the director in any case and will let you know what the outcome is.

Carl

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