CTWRUBEL Posted September 28, 2005 Share Posted September 28, 2005 Does anyone have experience with thawing, aliquoting and then re-freezing HPC's? We normally cryopreserve as soon as we get product and usually have it aliquoted before we get our yield results. In this case we had a great yield and it was stored in just two bags. The recipient got one and went into remission for almost 5 years. She is now relapsing and is up for a second transplant. There are enough cells in the remaining bag for another two transplants. So our director wants to know if there's any information on thawing, aliquoting and re-freezing product. Any data you have will be appreciated. Link to comment Share on other sites More sharing options...
kate murphy Posted October 5, 2005 Share Posted October 5, 2005 Carl, I have not heard or read of that being done before. Do you have any excess products from deceased pts you could try this on? Check viabilities pre/post for several aliquots. Progenitor cell assays will also tell you if cells are still viable. Interesting...can you let me know how it turns out? kate.murphy@bmc.org Link to comment Share on other sites More sharing options...
CTWRUBEL Posted October 7, 2005 Author Share Posted October 7, 2005 Good idea Kate. Unfortunately, we recently did a major purge of products collected from patients who are now deceased. We had added a clause to the HPC consent that allows us to discard unused product from deceased patients. We may have a few products left from collections done before the change was made to the consent. However, the program director is still waiting to hear back from next-of-kin to see if we can discard those products, so I don't think we could use those just yet.Our hematology lab does the viability studies but their results have been all over the place. I will bring it up to the director in any case and will let you know what the outcome is.Carl Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now