diloro Posted April 7, 2004 Share Posted April 7, 2004 Would like to speak with anyone working with stem cell processing, cryopreservation and transplants. Many facilities have Hematopoietic Progenitor Cell Processing Labs as part of their Blood Bank Dept. Would like to speak with someone who performs this service on a regular basis, to share questions and ideas. Thank You! Angie Link to comment Share on other sites More sharing options...
Cliff Posted May 1, 2004 Share Posted May 1, 2004 Hi diloro and welcome to the Donor Limits forums!I am part of a large facility, and we have close relationships with the stem cell and gene manipulation lab. One of our donors centers also collects PBPC.Feel free to post your questions, If I don't have the answers (and I probably won't :roll: ) then I'll contact some coworkers and have them post too. Link to comment Share on other sites More sharing options...
diloro Posted May 4, 2004 Author Share Posted May 4, 2004 Thank you, Clifford, for your response.Question: We perform sterility cultures on plasma-lyte A reagent used in stem cell processing and cryopreservation upon opening reagent and at end of week after processing patients. This is mandatory by FACT regulatory guidelines.We alo use ACDA as a prophylactic anitcoagulant before cryopreserving stem cells to decrease the risk of product clotting upon thaw at time of transplant.Many pts./donors are sensitive to ACDA during apheresis collection of stem cells and require Ca++ gluconate administration which causes binding (clots) if additional ACDA is not added to stem cells.There is not criteria that I know of that requires sterility testing of anticoagulant reagent involved in stem cell processing.Does anyone else in hematopoietic progenitor cell processing labs encounter this?If so, what criteria are they adhering to, if any. Link to comment Share on other sites More sharing options...
Laura Mc Posted July 14, 2004 Share Posted July 14, 2004 We only use Plasma-lyte and ACD-A bag once so we can avoid the whole sterility testing issue. I realize this may be wasteful but if you look at the cost of cultures etc it may be cheaper. Link to comment Share on other sites More sharing options...
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