bman2k Posted May 8, 2022 Share Posted May 8, 2022 (edited) We just started using the new Ortho Vision coming from manual tube method. We saw an increase of Anti-D positive (negative patient history) as the gel are more sensitive. We repeat the test with the now back-up tube method (negative result) and just report as negative for the anti-D. My question is, what is your blood bank policy in handling these discrepant result between the gel and the tube method? Do you just report as negative or do you change it to positive? Thank you. Edited May 8, 2022 by bman2k SbbPerson 1 Link to comment Share on other sites More sharing options...
Cliff Posted May 8, 2022 Share Posted May 8, 2022 Here is where we landed. SbbPerson 1 Link to comment Share on other sites More sharing options...
labguru Posted May 9, 2022 Share Posted May 9, 2022 We do not do the testing in tube to confirm. We will send the sample to American Red Cross for Genotyping for RHD variants if the reaction is 1-2+. The majority come back as weak D type 1 and are not considered to be at risk for production of allo-anti-D. It is generally accepted that females of child-bearing potential with weak D type 1 can be considered D positive for transfusion and are not candidates for Rh immune globulin. Their type will be updated with that comment. If the patient comes back as one of the other weak D types and there is a chance for production of the allo-anti-D, we will leave it as Rh negative and put the comment that testing was done in the patient history. AuntiS, Ensis01 and SbbPerson 3 Link to comment Share on other sites More sharing options...
AuntiS Posted May 10, 2022 Share Posted May 10, 2022 We do similar to labguru. We went from manual tube to automated gel (BioRad) and have found the same thing. If we have a discrepancy we send it out for genotyping. Most come back as a weak D type 1 (or 2 or 3). I am considering doing a manual tube type on our females less than 45 when they type as Rh positive the first time. And then sending out any discrepancies for genotyping. sandra SbbPerson 1 Link to comment Share on other sites More sharing options...
David Saikin Posted May 10, 2022 Share Posted May 10, 2022 When gel was our primary testing method a reaction w anti-D of 1+ or less was called Rh negative. I know of folks who call 3+ rxs or weaker Rh negative. This was determined by the Medical Director. SbbPerson 1 Link to comment Share on other sites More sharing options...
Mabel Adams Posted May 11, 2022 Share Posted May 11, 2022 For gel 2+ or less, we ask provider to allow us to send out for molecular typing if patient has childbearing potential. Otherwise, we usually interpret them as D positive but add a note that their type is weak and atypical so they may sometimes be reported as negative and other times (other places) as positive. If they have anti-D or some other reason (anti-C & anti-E?) we will choose to call them D neg. SbbPerson 1 Link to comment Share on other sites More sharing options...
cthherbal ☆ Posted May 11, 2022 Share Posted May 11, 2022 Regardless of gender, we do a weak D tube test on any patient who test at 2+ or below with the Ortho gel anti-D. If patients are possible weak D, we will transfuse with Rh negative as a precaution. SbbPerson 1 Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now