Ruling out "cold" antibodies with Gel Cards

[SbbPerson ☆]

Have people ever had to rule in or rule out cold antibodies based on reactions you get from a gel card panel/screen? 

For example in a case where you get a discrepant ABO result because a cold antibody is messing up the reverse typing. 

Anyone with any knowledge on this?  Thank you. 

Edited April 21, 2022 by sbbguy
brevity

[David Saikin]

Anti-M comes to mind most often, esp w Ortho gel cards.  It is possible to work up cold abs.  I've used the buffered gel cards to do so.   I've also used 2 stage papain pretreatment of panel cells (in gel). 

Edited April 21, 2022 by David Saikin
added more info

[Ensis01]

Gel likes colds, the larger IgM antibodies are more easily trapped in the columns matrix. You can only really identify cold antibodies with specificity (lots of anti-M) using Gel. For Panagglutination in screen, panel or a discrepant back type we resolve using tube.

[RichU]

We regularly used to run RT or even cold gel panels (LISS cells on Saline cards) to id M, P1, Le etc.

If suspected A2 patient with anti-A1, use A2 cells in the reverse grouping card.

We would test the grouping cells for the identified antibody.

WARNING! These may require a degree of skill as may have to perform manual testing.