SbbPerson ☆ Posted July 4, 2021 Share Posted July 4, 2021 Anyone here has any experience with saline IgG and it's use in detecting underlying alloantibodies? In a situation that may involve possible multiple alloantibodies or warm autoantibody(ies). I would like to find out more about the principle and the procedure behind this. Thank you. Link to comment Share on other sites More sharing options...
slsmith Posted July 5, 2021 Share Posted July 5, 2021 We use it when working with a patient with a warm antibody that has had all significant antibodies ruled out by the reference lab. The gel screen is still performed each time to make sure reactions are not getting stronger or no longer demonstrating. Then the saline panel is performed. We also transfuse with phenotypical matched blood for Kell, C, E and c . This procedure is usually being performed on the frequent fliers that we know are only coming to our hospital. We also use it when a gel shows no pattern, all cells positive or negative and we have gone to PEG and all cells are positive. The saline panel has to have at least 8 cells and ran with an auto control. If the panel is negative including the auto control it called a NSF and saline tech is use for the xm. IF the auto control is positive it is called a warm auto. If it is positive the work up is sent to the reference lab. The principle behind this I can't explain it is just what we do. SbbPerson 1 Link to comment Share on other sites More sharing options...
BloodBankBlake Posted July 5, 2021 Share Posted July 5, 2021 (edited) From my manager's "old school" approach that if a clinically significant antibody is present with a warm auto, it will react alone without enhancement media due to its supposed "high" titer. Similar to slsmith, we incorporate this approach when we have panagglutination in our more sensitive methodologies and are trying to "look through" these reactions. It's never our sole method or what we use to report, but just another arrow in the quiver of techniques. Edited July 5, 2021 by BloodBankBlake SbbPerson 1 Link to comment Share on other sites More sharing options...
SbbPerson ☆ Posted July 5, 2021 Author Share Posted July 5, 2021 1 hour ago, slsmith said: We use it when working with a patient with a warm antibody that has had all significant antibodies ruled out by the reference lab. The gel screen is still performed each time to make sure reactions are not getting stronger or no longer demonstrating. Then the saline panel is performed. We also transfuse with phenotypical matched blood for Kell, C, E and c . This procedure is usually being performed on the frequent fliers that we know are only coming to our hospital. We also use it when a gel shows no pattern, all cells positive or negative and we have gone to PEG and all cells are positive. The saline panel has to have at least 8 cells and ran with an auto control. If the panel is negative including the auto control it called a NSF and saline tech is use for the xm. IF the auto control is positive it is called a warm auto. If it is positive the work up is sent to the reference lab. The principle behind this I can't explain it is just what we do. Thank you for your post. I am sorry, but what is NSF? Do you know the name of the manufacturer of this saline panel? or know of any sources about saline IgG? Thank you for your time and help. Link to comment Share on other sites More sharing options...
SbbPerson ☆ Posted July 5, 2021 Author Share Posted July 5, 2021 38 minutes ago, BloodBankBlake said: From my manager's "old school" approach that if a clinically significant antibody is present with a warm auto, it will react alone without enhancement media due to its supposed "high" titer. Similar to slsmith, we incorporate this approach when we have panagglutination in our more sensitive methodologies and are trying to "look through" these reactions. It's never our sole method or what we use to report, but just another arrow in the quiver of techniques. Thank you for your response. What is the ingredient in this saline? Do you know the manufacturer name or the procedure steps? Thank you. Link to comment Share on other sites More sharing options...
Solution BloodBankBlake Posted July 5, 2021 Solution Share Posted July 5, 2021 5 hours ago, diplomatic_scarf said: Thank you for your response. What is the ingredient in this saline? Do you know the manufacturer name or the procedure steps? Thank you. We use regular blood bank saline or whatever suspension the reagent RBCs are in (as long as it isn't a LISS suspension). The procedure is just doing a screen/panel/xm don't add enhancement media, and incubate for 30min@ 37C SbbPerson 1 Link to comment Share on other sites More sharing options...
SbbPerson ☆ Posted July 6, 2021 Author Share Posted July 6, 2021 9 hours ago, BloodBankBlake said: We use regular blood bank saline or whatever suspension the reagent RBCs are in (as long as it isn't a LISS suspension). The procedure is just doing a screen/panel/xm don't add enhancement media, and incubate for 30min@ 37C This the tube method? Thanks so much ! How many tubes do you use for your panel ? Link to comment Share on other sites More sharing options...
SbbPerson ☆ Posted July 6, 2021 Author Share Posted July 6, 2021 Thanks everyone for your input! I found the method on the AABB website. It's attached if anyone wants to see it. Thanks again! METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf Link to comment Share on other sites More sharing options...
Kelly Guenthner Posted July 6, 2021 Share Posted July 6, 2021 If you're up for a little more discussion (and a little entertainment ) may I suggest this thread, from 2012? Saline incubation...why is this SOP still allowed? SbbPerson 1 Link to comment Share on other sites More sharing options...
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