DCeDCe Posted December 15, 2020 Share Posted December 15, 2020 Hi Blood Bankers, I have a strange question, is it possible to have an anti-Jka that does not react in a ficin panel?? If so, why? Any input on this is greatly appreciated! Thank you! Link to comment Share on other sites More sharing options...
exlimey Posted December 15, 2020 Share Posted December 15, 2020 Short answer: Yes How was the anti-Jka detected initially, i.e., what technique ? Assuming it was an antiglobulin test, but was it Gel, Solid Phase, LISS, PEG ? Yes, the panel cells are now ficin-treated and antibodies to Jka should be enhanced, but the test conditions that were used in the original assay may not exist when ficin-treated cells are used - one may need to add LISS or PEG, one may need to test by Gel or Solid Phase. Link to comment Share on other sites More sharing options...
John C. Staley Posted December 15, 2020 Share Posted December 15, 2020 Couple of more strange questions, how sure are you that it is anti-Jka? If you are sure, why were you doing a ficin panel? Can't wait to see what Malcolm has to say. David Saikin 1 Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted December 15, 2020 Share Posted December 15, 2020 (edited) I agree with exlimey. I once had an anti-E that reacted by LISS IAT, but try as we might, we could never get it to react with papain-treated red cells. We doubted the specificity, and so we sent it to Joyce Poole at the International Blood Group Reference Laboratory to have a look at it, and she confirmed both the specificity and the fact that it was non-reactive with papain-treated red cells. Anything is possible, as the antibodies refuse to read the damned text books! In the UK, it is almost a sine qua non that an "enzyme panel" is put up simultaneously with the IAT panel John, so I would almost certainly have done the same as DCeDCe. Edited December 15, 2020 by Malcolm Needs Spelling - or lack of! mrmic, John C. Staley, David Saikin and 3 others 6 Link to comment Share on other sites More sharing options...
exlimey Posted December 16, 2020 Share Posted December 16, 2020 23 hours ago, John C. Staley said: Couple of more strange questions, how sure are you that it is anti-Jka? If you are sure, why were you doing a ficin panel? Can't wait to see what Malcolm has to say. An EXCELLENT question, John, but as Malcolm suggests, is appears that there is still a large dose of "we always do that" in many laboratories. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted December 16, 2020 Share Posted December 16, 2020 2 hours ago, exlimey said: An EXCELLENT question, John, but as Malcolm suggests, is appears that there is still a large dose of "we always do that" in many laboratories. Ah, but don't forget that, not only did I work in a Reference Laboratory, but am also a self-confessed pedant, and so, if a patient was both c Negative and E Negative, and had an anti-c, we would go to the ends of the Earth to prove the presence or absence of an anti-E, as a matter of pride! David Saikin, exlimey and John C. Staley 3 Link to comment Share on other sites More sharing options...
exlimey Posted December 16, 2020 Share Posted December 16, 2020 1 hour ago, Malcolm Needs said: Ah, but don't forget that, not only did I work in a Reference Laboratory, but am also a self-confessed pedant, and so, if a patient was both c Negative and E Negative, and had an anti-c, we would go to the ends of the Earth to prove the presence or absence of an anti-E, as a matter of pride! I confess: I'm much the same way in the OCD sense. I'm also DCeDCe (R1R1), so perhaps this issue hits a little too close to the sensitive area. Malcolm Needs 1 Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted December 16, 2020 Share Posted December 16, 2020 1 hour ago, exlimey said: I confess: I'm much the same way in the OCD sense. I'm also DCeDCe (R1R1), so perhaps this issue hits a little too close to the sensitive area. I, too, am DCe/DCe (R1R1). This is getting spooky! Maybe you are on to something exlimey, and should write it up for Vox Sanguinis, Transfusion and/or Transfusion Medicine, or maybe even Nature! Link to comment Share on other sites More sharing options...
John C. Staley Posted December 17, 2020 Share Posted December 17, 2020 17 hours ago, Malcolm Needs said: I, too, am DCe/DCe (R1R1). This is getting spooky! Maybe you are on to something exlimey, and should write it up for Vox Sanguinis, Transfusion and/or Transfusion Medicine, or maybe even Nature! Thankfully I'm R1R2!!!! Malcolm Needs 1 Link to comment Share on other sites More sharing options...
exlimey Posted December 17, 2020 Share Posted December 17, 2020 18 hours ago, Malcolm Needs said: I, too, am DCe/DCe (R1R1). This is getting spooky! Maybe you are on to something exlimey, and should write it up for Vox Sanguinis, Transfusion and/or Transfusion Medicine, or maybe even Nature! An interesting idea, but I'm not sure I want my psychological dirty laundry hung out for the world to see. Malcolm Needs 1 Link to comment Share on other sites More sharing options...
exlimey Posted December 17, 2020 Share Posted December 17, 2020 1 hour ago, John C. Staley said: Thankfully I'm R1R2!!!! Sorry, John. You'll have to start your own club. Perhaps "f-negatives Rule", or something like that. Malcolm Needs and John C. Staley 2 Link to comment Share on other sites More sharing options...
David Saikin Posted December 17, 2020 Share Posted December 17, 2020 1 hour ago, John C. Staley said: Thankfully I'm R1R2!!!! Me too John Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted December 17, 2020 Share Posted December 17, 2020 1 hour ago, exlimey said: Sorry, John. You'll have to start your own club. Perhaps "f-negatives Rule", or something like that. But don't forget that those of us who are DCe/DCe are ALSO f Negative! Link to comment Share on other sites More sharing options...
exlimey Posted December 17, 2020 Share Posted December 17, 2020 10 minutes ago, Malcolm Needs said: But don't forget that those of us who are DCe/DCe are ALSO f Negative! Good point, but we're very unlikely to make the antibody. Link to comment Share on other sites More sharing options...
exlimey Posted December 17, 2020 Share Posted December 17, 2020 2 hours ago, John C. Staley said: Thankfully I'm R1R2!!!! 59 minutes ago, David Saikin said: Me too John See....you've already got the makings of a club. You can celebrate your CEce heterozygosity together. David Saikin and John C. Staley 1 1 Link to comment Share on other sites More sharing options...
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