Blood_Banker Posted July 31, 2020 Share Posted July 31, 2020 (edited) There is a donor that reacts with two polyclonal anti-s antisera from 2 different manufacturers but not a monoclonal anti-s (contains clone P3BER). Is this likely to be an s antigen variant? Quite sure that the donor was genotyped as s positive which is the only reason we tested with polyclonal antisera after we got a negative result with the monoclonal anti-s. Donor is Mi(a+). Anybody seen anything similar? Thanks in advance. Edited July 31, 2020 by Blood_Banker Link to comment Share on other sites More sharing options...
exlimey Posted July 31, 2020 Share Posted July 31, 2020 Yes. In the examples I've seen, the usual the culprit is a gene re-arrangement that results in expression of the Dantu antigen. If I remember correctly, the P3BER clone does not react with Dantu+ cells. If it isn't mentioned in the Directions for Use, you could check with the technical people at Millipore/Bioscot. The presence of "Mia" (an obsolete umbrella term that can apply several "Miltenberger" antigens), already indicates that some MNS gene shuffling has occurred. Blood_Banker 1 Link to comment Share on other sites More sharing options...
Baby Banker Posted July 31, 2020 Share Posted July 31, 2020 2 hours ago, exlimey said: Yes. In the examples I've seen, the usual the culprit is a gene re-arrangement that results in expression of the Dantu antigen. If I remember correctly, the P3BER clone does not react with Dantu+ cells. If it isn't mentioned in the Directions for Use, you could check with the technical people at Millipore/Bioscot. The presence of "Mia" (an obsolete umbrella term that can apply several "Miltenberger" antigens), already indicates that some MNS gene shuffling has occurred. I was going to suggest that you check with the manufacturer. If you haven't already, look at the package insert. Blood_Banker 1 Link to comment Share on other sites More sharing options...
Blood_Banker Posted July 31, 2020 Author Share Posted July 31, 2020 Thanks. Finally found a great journal article about this phenomena - just takes googling the right keywords to find something. Here is a link for those interested. https://onlinelibrary.wiley.com/doi/full/10.1111/vox.12909 JasonS and exlimey 1 1 Link to comment Share on other sites More sharing options...
exlimey Posted August 3, 2020 Share Posted August 3, 2020 On 7/31/2020 at 4:42 PM, Blood_Banker said: Thanks. Finally found a great journal article about this phenomena - just takes googling the right keywords to find something. Here is a link for those interested. https://onlinelibrary.wiley.com/doi/full/10.1111/vox.12909 Oops. Perhaps "Mia" is not as obsolete as I believed. Great article/reference. Link to comment Share on other sites More sharing options...
e specificity Posted August 3, 2020 Share Posted August 3, 2020 We had a situation with discordant/variable little s typings (but caucasian) and genotyping came back without flags as little s+. In the end, the patient had a glycophorin hybrid that required sequencing to determine with an antibody classified as anti-Ena. Link to comment Share on other sites More sharing options...
e specificity Posted August 3, 2020 Share Posted August 3, 2020 To add, Reid's The blood Group Antigen FactsBook 3rd ed. MNS system pages has a nice breakdown of the "Miltenberger" phenotypes for the hybrids. Link to comment Share on other sites More sharing options...
exlimey Posted August 3, 2020 Share Posted August 3, 2020 18 minutes ago, e specificity said: We had a situation with discordant/variable little s typings (but caucasian) and genotyping came back without flags as little s+. In the end, the patient had a glycophorin hybrid that required sequencing to determine with an antibody classified as anti-Ena. Eek ! I hope they don't need transfusion. Link to comment Share on other sites More sharing options...
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