Jump to content

Recommended Posts

There is a donor that reacts with two polyclonal anti-s antisera from 2 different manufacturers but not a monoclonal anti-s (contains clone P3BER). Is this likely to be an s antigen variant? Quite sure that the donor was genotyped as s positive which is the only reason we tested with polyclonal antisera after we got a negative result with the monoclonal anti-s. Donor is Mi(a+). Anybody seen anything similar? Thanks in advance. 

Edited by Blood_Banker
Link to comment
Share on other sites

Yes. In the examples I've seen, the usual the culprit is a gene re-arrangement that results in expression of the Dantu antigen. If I remember correctly, the P3BER clone does not react with Dantu+ cells. If it isn't mentioned in the Directions for Use, you could check with the technical people at Millipore/Bioscot.

The presence of "Mia" (an obsolete umbrella term that can apply several "Miltenberger" antigens), already indicates that some MNS gene shuffling has occurred.

Link to comment
Share on other sites

2 hours ago, exlimey said:

Yes. In the examples I've seen, the usual the culprit is a gene re-arrangement that results in expression of the Dantu antigen. If I remember correctly, the P3BER clone does not react with Dantu+ cells. If it isn't mentioned in the Directions for Use, you could check with the technical people at Millipore/Bioscot.

The presence of "Mia" (an obsolete umbrella term that can apply several "Miltenberger" antigens), already indicates that some MNS gene shuffling has occurred.

I was going to suggest that you check with the manufacturer.  If you haven't already, look at the package insert.  

Link to comment
Share on other sites

On 7/31/2020 at 4:42 PM, Blood_Banker said:

Thanks. Finally found a great journal article about this phenomena - just takes googling the right keywords to find something. Here is a link for those interested.

https://onlinelibrary.wiley.com/doi/full/10.1111/vox.12909

Oops. Perhaps "Mia" is not as obsolete as I believed.:) Great article/reference.

Link to comment
Share on other sites

18 minutes ago, e specificity said:

We had a situation with discordant/variable little s typings (but caucasian) and genotyping came back without flags as little s+. In the end, the patient had a glycophorin hybrid that required sequencing to determine with an antibody classified as anti-Ena.  

Eek ! I hope they don't need transfusion.

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
  • Recently Browsing   0 members

    • No registered users viewing this page.
  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.