Sharon Ann Posted March 10, 2020 Share Posted March 10, 2020 I would like to hear what positive and negative controls are being set up when you do a titer on pregnant patients. Are if any controls are being done at all. Thanks! Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted March 10, 2020 Share Posted March 10, 2020 If the titre is on a new patient, we (UK) would a NIBSC (National Institute for Biological Standards and Control) anti-D. If it is a second or subsequent sample, we would titrate the stored (and frozen) previous sample, in parallel with the new sample. If there was found to be a difference in titre, we would again use a NIBSC anti-D in parallel. Link to comment Share on other sites More sharing options...
John C. Staley Posted March 10, 2020 Share Posted March 10, 2020 If I remember correctly, back in ancient times, we did not include any control with the initial titer. (Probably because no one thought of it!) For any subsequent titers we would use a frozen sample from the previous titer. Malcolm Needs 1 Link to comment Share on other sites More sharing options...
Ward_X Posted March 11, 2020 Share Posted March 11, 2020 My facility's previous control was running the previous sample in tandem and the results had to agree within one dilution. This process is currently being remodeled. Ensis01 1 Link to comment Share on other sites More sharing options...
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