When would a cold antibody not be detected at IS?

[pathlabtech]

Here's the antibody screen:

Cell	IS	37C	AHG
SC 1	neg	neg	1+
SC 2	neg	neg	1+
Auto	neg	neg	neg

The possible interpretations are:

1. single warm antibody, antigen present on both cells

2. antibody to high prevalence antigen

3. complement binding by a cold antibody not detected at IS

Can someone explain interpretation #3? 

 

 

 

[exlimey]

6 hours ago, pathlabtech said:

Here's the antibody screen:

Cell	IS	37C	AHG
SC 1	neg	neg	1+
SC 2	neg	neg	1+
Auto	neg	neg	neg

The possible interpretations are:

1. single warm antibody, antigen present on both cells

2. antibody to high prevalence antigen

3. complement binding by a cold antibody not detected at IS

Can someone explain interpretation #3? 

 

 

 

Interpretation #3 should only be considered if a polyspecific antiglobulin reagent was used, i.e., a "complement coat" will only be detected if the antiglobulin reagent used contains an anti-complement component. Even if that is the case, Interpretations #1 and #2 are far more likely.