Centriguge speeds

[Blood_Banker]

If you spin a sample for 20 secs at 500g, is this the equivalent of spinning a sample for 10 secs at 1000g?

 

We typically use 500g and 1000g on our centrifuges but the new cell washer we have purchased has a high and low speed. The low speed is lower than 500g and the high speed is higher than 1000g. I guess im trying to understand if I can somehow calculate how long I spin at the speeds of the new cell washer that will be equivalent for spinning at 500g and 1000g for different times.

Edited August 16, 2019 by Blood_Banker

[SMILLER]

Whatever centrifuge or cell washer you use, you need to check it at different times for each speed.  What you want to see is how long it takes to get a clearly delineated cell button for both a positive and negative reactions, in addition to a negative that dissolves easily. 

We check ours yearly, (I think it's regulated by our inspector-JCAHO).  And sometimes we find we have to adjust a time for -- say -- an IS -- from 15 to 20 secs or whatever.

Scott