Jump to content

DAT - ACCEPTABLE METHODS FOR PERFORMANCE


lpregeno

Recommended Posts

Our Blood Bank has always performed the DAT by tube method (the exception being cord bloods). We do the Poly and if it is positive, then we do the IgG and complement. Recently a patient presented in the ER 5 days post-transfusion with a 4.2 HGB and a positive antibody screen. His autocontrol was positive as were most of the panel cells. His Poly DAT was negative. Due to the patient's symptoms I still highly suspected a delayed transfusion reaction. Our reference lab agreed to see what they could figure out. They only perform the IgG DAT and they perform it in gel. They got a strong 1+ and their eluate showed an Anti-e.

Needless to say, this caused me to doubt our previous method of performing these tests. I was able to validate performing non-cord blood samples on our Erytra and would prefer for this to be our main method for doing these. I previously was looking into getting rid of the complement portion by default (i.e. Poly - Pos, IgG - Neg) because we use so little of it that it is a big waste of money. We often throw out complement check cells that have never been opened. However, now I am looking at getting rid of the Poly and just doing the automated (gel) IgG and tube complement. I see my choices could be  the following:

1. Eliminate the Poly and perform the automated IgG and tube complement on all DAT samples

2. Perform the automated IgG and, if positive, perform the tube Poly and default the complement

I want to be sure that my decision is acceptable to the AABB and CAP, hopefully save some money for our department, and still maintain proper patient care that the physicians will be happy with.

Any thoughts and/or suggestions on this would be greatly appreciated!

Link to comment
Share on other sites

We dropped the poly for the same reasons you are considering.  We have an Echo and perform ours on there and have tube reagents for backup or in case the sample is too small to run on the Echo.  We are AABB accredited, but not CAP (we use HFAP) and our processes have not been questioned by either agency.

Link to comment
Share on other sites

  • 2 weeks later...
  • 3 weeks later...

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
  • Recently Browsing   0 members

    • No registered users viewing this page.
  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.