jojo808 Posted April 9, 2019 Share Posted April 9, 2019 Let's try this again. Anyone using negative controls for their backtype reagents (A1 cell and B cells)?? If so, what are you using? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted April 9, 2019 Share Posted April 9, 2019 Anti-A for the group B red cells and anti-B for the A1 red cells. jojo808, Dansket and bbslm 2 1 Link to comment Share on other sites More sharing options...
ANORRIS Posted April 9, 2019 Share Posted April 9, 2019 ORTHO CONFIDENCE John C. Staley, jojo808 and JasonS 2 1 Link to comment Share on other sites More sharing options...
John C. Staley Posted April 9, 2019 Share Posted April 9, 2019 I'm pretty sure any of the commercial QC systems will have this covered and I don't remember them being all that expensive. I have used both the Ortho and Immucor systems and don't remember any issues. ANORRIS, jojo808 and Malcolm Needs 2 1 Link to comment Share on other sites More sharing options...
Kathyang Posted April 9, 2019 Share Posted April 9, 2019 We use saline just to make sure they aren't just agglutinating. jojo808 1 Link to comment Share on other sites More sharing options...
pinktoptube Posted April 10, 2019 Share Posted April 10, 2019 16 hours ago, Malcolm Needs said: Anti-A for the group B red cells and anti-B for the A1 red cells. Same or QC material from Quotient or BioRad works jojo808 1 Link to comment Share on other sites More sharing options...
NicolePCanada Posted April 11, 2019 Share Posted April 11, 2019 We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive. Texas Lynn and jojo808 1 1 Link to comment Share on other sites More sharing options...
Karen knight Posted April 12, 2019 Share Posted April 12, 2019 I also use Anti-B for affirmagen A1 rgt neg ctl and Anti-A for affirmagen B rgt neg control as part of new lot/newshipment check. as part of daily control I use ortho confidence jojo808 1 Link to comment Share on other sites More sharing options...
Bb_in_the_rain Posted April 12, 2019 Share Posted April 12, 2019 Ortho Confidence jojo808 1 Link to comment Share on other sites More sharing options...
SMILLER Posted April 15, 2019 Share Posted April 15, 2019 Albumin. Scott jojo808 1 Link to comment Share on other sites More sharing options...
AMcCord Posted April 15, 2019 Share Posted April 15, 2019 Plasma from an AB patient (negative antibody screen, no issues w/ blood type). jojo808 1 Link to comment Share on other sites More sharing options...
BankerGirl Posted April 15, 2019 Share Posted April 15, 2019 On 4/11/2019 at 9:09 AM, NicolePCanada said: We use Immucor for our reverse (back) type and the package insert indicates that we need to confirm the reactivity of the A1, A2, and B red blood cells, not the negative results. So, we only daily QC that they are reactive. We do the same. Texas Lynn 1 Link to comment Share on other sites More sharing options...
David Saikin Posted April 19, 2019 Share Posted April 19, 2019 If I am qcing gel - I use the diluent as a neg control. If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc. (personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA). jojo808 1 Link to comment Share on other sites More sharing options...
SMILLER Posted April 20, 2019 Share Posted April 20, 2019 22 hours ago, David Saikin said: If I am qcing gel - I use the diluent as a neg control. If I am qcing tube reagents, I only test a pos and neg w anti-D, otherwise, only positive qc. (personally, I think that tubes should be qc'd pos and neg but it is not required in the US (FDA). Hmmm. Here in Michigan, we are indeed doing negative controls for reverse cells (we just use albumin). We are FDA and JCAHO inspected. Scott jojo808 1 Link to comment Share on other sites More sharing options...
Dansket Posted April 20, 2019 Share Posted April 20, 2019 On 4/9/2019 at 2:16 AM, Malcolm Needs said: Anti-A for the group B red cells and anti-B for the A1 red cells. By definition, reagent reverse grouping A1 and B cells are used to detect anti-A and anti-B antibody in patient plasma. Accordingly A1 cells should react with anti-A but not with anti-B, and B cells should react with anti-B but react with anti-A. Therefore, A1 cells should not be agglutinated by anti-B. No agglutination is a negative test result, i.e., a negative control test. Likewise, B cells should not be agglutinated by anti-A. No agglutination is a negative test result, i.e. a negative control test. Testing A1and B cells with AB plasma, Diluent, Albumin or saline may demonstrate that the test cells are not spontaneously agglutinating in their presence which serves as a negative control for those reagents, but does not serve as a negative control test for A1 cells or B cells. Ensis01, jojo808 and Malcolm Needs 3 Link to comment Share on other sites More sharing options...
slsmith Posted April 22, 2019 Share Posted April 22, 2019 Interesting question....maybe we should be but not we don't Link to comment Share on other sites More sharing options...
Laurie Underwood Posted April 24, 2019 Share Posted April 24, 2019 We use Ortho Confidence antisera for the positive QC. Anti-A for the group B red cells and anti-B for the A1 red cells for the negative QC. jojo808 1 Link to comment Share on other sites More sharing options...
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