Jump to content

CORD BLOOD NOT MATCHING HEEL STICK TYPE


Recommended Posts

We had a case where we got O negative on the cord blood sample and O positive on a heelstick of the baby. Results were rechecked and were the same. Results were carried out through IgG. Mom is O negative. The sample is labeled with the correct label. Besides the nurse collected the wrong patient, any ideas why this happened.

Link to comment
Share on other sites

If you put a drop of blood on something like a filter paper, and then add a drop of 1M NaOH, if it is adult blood, after a couple of minutes it will turn a sort of yellow/brown colour, as the Hb is denatured by the alkaline, whereas, if it is blood derived from the baby (including cord blood), the red cells will stay red, as HbF is not denatured by the alkaline for much longer.

It is rather like doing a Kleihauer, but by "bucket chemistry", as it is known!  :unsure::unsure::unsure::unsure::unsure:

Link to comment
Share on other sites

2 hours ago, Malcolm Needs said:

If you put a drop of blood on something like a filter paper, and then add a drop of 1M NaOH, if it is adult blood, after a couple of minutes it will turn a sort of yellow/brown colour, as the Hb is denatured by the alkaline, whereas, if it is blood derived from the baby (including cord blood), the red cells will stay red, as HbF is not denatured by the alkaline for much longer.

It is rather like doing a Kleihauer, but by "bucket chemistry", as it is known!  :unsure::unsure::unsure::unsure::unsure:

Nice !!! Old School.B)

Link to comment
Share on other sites

3 hours ago, Malcolm Needs said:

If you put a drop of blood on something like a filter paper, and then add a drop of 1M NaOH, if it is adult blood, after a couple of minutes it will turn a sort of yellow/brown colour, as the Hb is denatured by the alkaline, whereas, if it is blood derived from the baby (including cord blood), the red cells will stay red, as HbF is not denatured by the alkaline for much longer.

It is rather like doing a Kleihauer, but by "bucket chemistry", as it is known!  :unsure::unsure::unsure::unsure::unsure:

Works well when someone brings you a 'red' diaper or burp rag and wants to know if you can do a blood type to see if the blood is mom's or baby's.

(Doesn't work worth a darn though for cherry/red colored meds or drinks :rofl:)

Link to comment
Share on other sites

1 hour ago, AMcCord said:

Works well when someone brings you a 'red' diaper or burp rag and wants to know if you can do a blood type to see if the blood is mom's or baby's.

(Doesn't work worth a darn though for cherry/red colored meds or drinks :rofl:)

I knew this sounded familiar!  

Link to comment
Share on other sites

8 hours ago, Malcolm Needs said:

If you put a drop of blood on something like a filter paper, and then add a drop of 1M NaOH, if it is adult blood, after a couple of minutes it will turn a sort of yellow/brown colour, as the Hb is denatured by the alkaline, whereas, if it is blood derived from the baby (including cord blood), the red cells will stay red, as HbF is not denatured by the alkaline for much longer.

It is rather like doing a Kleihauer, but by "bucket chemistry", as it is known!  :unsure::unsure::unsure::unsure::unsure:

We do this test on all Rh (weak D) negative cord blood specimens from babies with Rh negative moms -- just to make sure that it is baby blood and that mom doesn't need RhIG.

Link to comment
Share on other sites

What was the DAT result?  If it was weakly or 1+ positive, you cannot accept the Rh result. At our facility, if the mom is Rh Neg and the baby initially types Rh Neg, with the DAT positive, we cannot perform the weak D; we have to send it out to the Reference Lab for testing.  If the antigen sites for D are coated already by anti-D, then the baby might type as Rh Neg. The cells need to be eluted,  EGA can be used to knock off any antibody and do the blood typing.  If the doc doesn't want the baby stuck, we turn out Rh Indeterminate, and recommend the baby be typed later.

Link to comment
Share on other sites

Ok, first, DAT was negative. I did the NaOh test, we only had 0.1 N. I ran a cord and adult for comparison.The cord in question did look more like an adult sample then a cord sample. Mother is O neg, so maybe it was her blood?

 It seems to me, a long time ago, in a lab far away, we would heelstick the babies if we got Rh negative and the mom was Rh negative. To make sure the baby's type was correct and if mom needed RhIg or not.

Link to comment
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
  • Recently Browsing   0 members

    • No registered users viewing this page.
  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.