Jump to content
Sign in to follow this  
studenttttttt

Why don't we incubate in DAT testing?

Recommended Posts

The DAT is a test to detect either antibodies or complement (or both) on the surface of the red cells that has sensitised the red cells in vivo.  Therefore, in a way, the red cells have already been incubated within the body.  There is, therefore, no need to incubate ex vivo or in vitro, whichever way you want to put it.

Share this post


Link to post
Share on other sites

It shouldn't cause a false positive, because the plasma would be from the patient, as would the red cells, and so they would be from the same source.  However, it is possible that the reaction may be enhanced by more antibody sensitising the red cells, but this would not be a false positive.

Share this post


Link to post
Share on other sites

If one is doing a DAT in order to determine the status of a possible acute transfusion reaction, then the presence of antibodies on donor cells is the concern.  There may be some use in incubation here to enhance uptake, but I have never heard of it being part of anyone's transfusion work-up procedure.

Scott

Share this post


Link to post
Share on other sites

Incubating patient plasma with patient red blood cells and then applying the antiglobulin test is no longer a Direct Antiglobulin Test but an Autocontrol test which is an Indirect Antiglobulin Test.  Some may think an Autocontrol test gives the same results as a Direct Antiglobulin Test, but that is not always true.

 

Share this post


Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now
Sign in to follow this  

  • Advertisement

×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.