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CAMLT

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    BLOODBANK

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  1. If the auto control in gel is positive, there is high chance that the other screening cells reactions in gel also positive, strength of variation may be possible. Since gel is more sensitive to warm auto, tube test including DAT may not catch. I will switch to LISS screen (not PEG) to see if screen is still positive. Sometime even DAT negative which normally not justifies to do eluate, but if you really perform the elution, there may have positive reactions across over the panel since elution tends to be more sensitive to catch warm auto. This is why to call a pt has warm, the eluate must have positive reactions as a minimum. WAA is not regarded as clinically significant, but interfering routine tests. For the future reference, you can put a comment in this pt folder to say like use LISS to do antibody screen. If WAA strong enough, auto/allo adsorption may be needed. In that case, the DAT will be surely positive.
  2. CAMLT

    Tango Optimo

    I agree. It is all about sensitivity. Any AB titter/strength less than the certain level, the analyser will not pick up. That is one factor to contribute the claim that transfusion is associated with risk for pt. Now, However, it is hard to get infor about the testing sensitivity for the reagents we use. Maybe it is because they cannot control the pt side since each pt is different. Now, if the company did not have a clue about testing sensitivity about their antisera, how can they be sure their reagents are potent enough in the real world? Well even they can prepare their controls to validate, but how low titter can go for these controls so they can be sure their antisera are potent enough? There must have some kind of guide line there for each company to make these reagents, but does FDA have this guideline?
  3. I am looking for the BIO-RAD TANGO OPTIMO USER GUIDE. Anyone can provide a web link or post to the library on PathTalk? My lab has a hard copy, but I want to have an electronic copy for myself so I can use it any time I want. Thanks.
  4. Welcome to the forums CAMLT :)

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