Carina
Content Type
Store
Profiles
Forums
Blogs
Events
Frequently Asked Questions
Gallery
Downloads
Glossary
Links Directory
Questions
Jobs
Vendors
Everything posted by Carina
-
Confirming Weak A or B by adsorption elution
Carina replied to Carina's topic in Transfusion Services
Thank you Malcom very helpful. We will do the same Incubate 4C, perform Lui elution. Incubate at room temperature and test against A1-, B-, and O -cells. I was unable to log in the website for a while, hence the long response time. I changed my personal data, which created the minor issue. -
Could someone share their procedure for confirmation of weak A or B antigen and antibody by adsorption elution. Specifically controls, how to make sure that the findings are weak A or B and not something else. Thank you a lot
-
Did anyone validate their DTT treatment of red cells procedure? Any advise with the validation protocol.
-
Cathy how did you solve the PBS pH 8.0 issue? I have the same problem today. I have DDT power to prepare the reagent to treat RBCs, however no PBS at the 8.0 pH and no solutions to adjust pH. I cannot find any supplier for the PBS at that pH.
-
Yes it was.
-
We use BioRad DiaCidel elution. There is no mentioning of non-specific uptake of IgG. The anti -K in the eluate is same strenth (2+) as in plasma.
-
Try to get my head around these tricks.
-
Thank you Malcolm for the quick reply. I was thinking that as well, but not sure how to prove the case. The patient's surely have alloanti -E or -K as well, for these were known long before the eluate showed the antibody specificity.
-
What is the possible cause? Patient has a known history of anti -E, always receiving crossmatch compatible E negative RBCs. Patient's phenotype is E negative. However anti -E is eluted from the red cells. I have seen at least three different patients with known anti -E with episodes of anti -E eluted from the red cells. The most recent patient is known K, also having episodes of K in the eluate. - the antibodies in the plasma E or K are strong 2+ reactive, no possible to miss in the crossmatch. We do full gel crossmatch -the donor units are phenotyped both in the donor center and then again prior to crossmatch in transfusion center.
