Please understand that NO ONE method is guaranteed to detected 100% of the antibodies 100% of the time. We have 2 automated machines, 2 semi-automated machines and plain old fashion manual tube testing. We have seen, REPEATEDLY, where one method would detect an antibody while the other one would not. If your instrument failed to call a pos. a pos. then yes there is a problem. If it failed to detect the antibody, then as Anna said, you have to evaluate other possible issues and possibly chalk it up to methodology. Good luck.