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About applejw1

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    Paso Fino horses, dogs, cats and Blue and Gold Macaws
  • Biography
    Graduated from University of Florida with BS in Medical Technology
    SBB 1993
  • Location
    South Carolina
  • Occupation
    Medical Technologist - Blood Bank
  • Real Name
    Jeanne Towery

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  1. Blood Bank Regulations

    My best advice is to know where to find the regulatory requirements - being the Supervisor, in my opinion, is functioning as a resource and a leader for your techs. Trying to memorize everything is probably not the best use of your time - and the interviewers certainly have not memorized all of the standards - focus more on where the department is strong and where the weaknesses are and come up with a plan on how to enhance the strengths and improve the weaknesses. Learn, network, and learn some more. Good luck!
  2. None of the facilities that I have been affiliated with (all tertiary care >500 bed hospitals) have taken the irradiator device out of service waiting on dosimetry testing. Every blood product that is irradiated has an exposure indicator - it was my understanding that dosimetry verified that appropriate amount of radiation reaches all areas of the canister where there could be a blood product.
  3. Elution Studies

    When do you perform an elution? (e.g. all positive DATs, all positive DATS within 3 months of transfusion, IgG positive only) Positive DAT with Anti-IgG once every 30 days What method is utilized for the elution? Gamma EluKit II What method is utilized for testing the eluate? Gel or modified AHG- Tech preference. How is the eluate tested? (e.g. screening cells, full panel, specially selected cells) Full panel Feel free to mention any special notes/criteria for which I may not have though to ask. Testing eluates in gel has been more helpful in identifying patients with low titer warm autoantibodies. Caution must be taken when testing to remove stroma from eluate to avoid ambiguous reactivity.
  4. Microwave plamsa thawer

    To answer Lisa E's question, 5-day plasma is labeled as Thawed Plasma and expires 5 days from the date of thaw at 23:59 of Day 5. Even if it starts as FFP, once it is labeled as Thawed Plasma it has a 5 days expiration period.
  5. Microwave plamsa thawer

    I just read a warning letter from the FDA directed toward the manufacturer of ARK BIO Plasma defroster in Canada. Apparently, as of 3/7/17, they were warned against marketing a medical device without FDA clearance. Earlier versions were cleared but the have since "adulterated" the device which requires new submissions for clearance. I have never used the device but am looking into something water-less due to the problems of potential microbial growth in water (even with an additive).
  6. Immucor W.A.R.M.

    Has anyone ever had patient plasma coagulate after 2x W.A.R.M autoadsorption? Specimen was stored at room temperature and looked gelatinous after overnite.
  7. I am searching for a portable datalogger to be used in a portable small blood storage cooler. Most of the forum postings refer to companies that are no longer in business or have been absorbed into larger companies. If anyone is using a datalogger to record temperatures in a remote cooler (not a refrigerator) that uses a probe or vial that would fit in a small space with 3 units of packed red cells, could you please share? I am looking specifically at a Pelican Biothermal ProMed Credo 472 or Cool Cube 3.5L where the payload space is approximately 6x6x6.
  8. For anyone that may substitute Liquid Plasma for Thawed Plasma, please be aware that there is a risk of transfusion-associated GVHD with Liquid Plasma because of viable lymphocytes in this product. If you transfused it to someone who is at risk for GVHD, it could be life-threatening.
  9. The Hemo Bioscience 0.2 M DTT comes in 2ml vials - the ratio listed in the Technical Manual is a 1:4 ratio - 1 drop of packed red cells to 4 drops of 0.2M DTT. Incubate at 37 and then wash at least 4 times with 0.9% saline.
  10. FFP thawing with no hot water

    To thaw FFP,Cryo,etc... standards allow a temperature range of 30-37C. Cold water would not meet that requirement even if it did eventually thaw the product. Is there a water bath that you could buy or borrow (and validate) before your hot water is turned off? My recommendation is that if you are thawing blood components intended for transfusion, you should have a reliable process and water in a bucket is a little iffy on the standard of practice. I have done it myself but that was 30 years ago when standards weren't as specific about temperature ranges and did not include a low temperature.

    Thank You, Great Webmaster! You don't have any idea how good smashing things is!
  12. Expired Panel Cells

    We have a fairly large inventory of expired panels and screening cells because we have students and see a large number of antibody problems - some of which require using an expired cell to exclude the occasional antibody - that being said, we do as Mollyredone described - we test the particular cell with antisera to demonstrate that the antigen is still demonstrating on the cell if the cell will be used to exclude that particular antibody (most frequently it is S in a patient with anti-M). How is CLIA regulating IRL that have extensive panels of frozen cells (definitely not following manufacturer's instructions for storage or preparation). I have not seen a reference report yet in my career that includes the disclaimer that the lab did off-label testing with expired/frozen-thawed reagent red cells - but I know when they report that an anti-Ge3 or anti-Vel was identified in the patient's sample that rare frozen-thawed cells must have been used at some point in the investigation, right?
  13. I'm not certain what "batch treatment" means..... if it refers to treating several cells for testing at the same time, then Yes - we are treating screening cells (1-3) and K-negative donor red cells for compatibility testing to be used on a current sample. If it refers to treating a panel of cells to keep for an extended period of time (like longer than 12 hours) to use for future patient sample testing, then No - and my limited experience with this says that the reagent red cells (especially - donor cells fared slightly better) become fragile after treatment even when washed 4-6x and lyse over time rendering them useless for testing.
  14. Strange Blood Type Discrepancy

    Brenda, You should do a poster on this - it is really interesting and I think would be very educational for newer techs, smaller hospitals that may not encounter such an interesting patient. Thanks for posting it!
  15. I haven't seen any responses that address the issue of the current antibody screen status - negative or positive. It was my understanding that part of the Truth Table for Electronic XM should include a current antibody screen (irregardless of antibody history). If the current screen is positive, I don't believe the patient should qualify for electronic compatibility testing.