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Bb_in_the_rain

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Everything posted by Bb_in_the_rain

  1. We have had some colds, non-reactive in solid phase but reactive in tube. In above case, I would approach it by performing antibody identification by tube method. (since I am conservative)
  2. Since it is widely publicized to consider Weak D Type 1,2 or 3 as D+, I think it will be a good idea to document anti-D production in Weak D type 1,2 or 3; at least as an abstract to professional organization if not as a full case report. Each case may count as "exception" and if there were too many exceptions, the hypothesis (or proven theory) may be challenged. That is just "my feeling" anyways. I can be very wrong since I am not an expert .
  3. Since we have capability for molecular tests in this lab, we would refer to molecular testing for the following populations, (if D typing is <2+) 2) Women with child-bearing potentials 3) Potential transplant recipients If you do not have molecular testing capability, I suppose you can call them D- (to be conservative) For the following population of patients, we interpret any positive reaction as D+ (if the DAT is negative) 1) Males (<18 year old) (exception is our local Children's Hospital) 2) Female with no child bearing potential 3) blood or organ donors
  4. A powerpoint slide from 2018 AABB case presentation by Woo JS, et al. included a page of reported anti-D cases (see attached picture) So there would be 7 total cases of anti-D production in Weak D type 1,2, and 3, including the case that was presented. The authors also have footnote that some of these anti-D has both auto- and allo- antibody characteristics.
  5. That will be my assumption as well. When I read up "Olsson M, et al. The Fyx phenotype is associated with a missense mutation in the Fyb allele Preductin Arg89Cys in Duffy glycoprotein.", authors explictly did not mentioned that individuals with Fya/Fyx does not produce anti-Fyb. That lead me to second guess myself and look for case reports but could not find any.
  6. Digitonin is the only thing I have heard of as well.
  7. If Fyx allele is identified in donor, we consider this as Fy(b+) since it can elicit antibody product in the recipients. My question is- Are patients with Fyx allele capable of producing anti-Fyb? Are there any case reports?
  8. That may be because she missed the 4th year of college where she has to start thinking about what to do after she finish school and real life situations.
  9. The fact that the baby is DAT negative makes me question whether it is really due to anti-A1. You mentioned that this baby has infection, right? I am wondering if this can be related to infection or antibiotic. I also start thinking if the baby's red cells can be polyagglutinatable.
  10. But we can pass SBB without brushing up on terminology. This is a different beast lol
  11. Interesting!! It will be a fun case study for us to learn from if you would be kind enough to post up this case on this site!
  12. You all are so lucky to have direct access to such lab!
  13. I completely agree with you with Weak D Type 1, 2 and 3. I think there are more than 3 cases of allo-anti-D so far. I am questioning the approach to give D+ blood to these patients as well (personally opinion, I know it may be an ongoing debate). Let me look up the case reports of anti-D in Weak D Type 1,2,or 3 to get back to you. I have a feeling that 3 is a bit too little. My other question is whether to look for other D variants when Weak D type 1,2,or 3 were detected in individuals of African origin as they may have other additional variants that can potentially lead to production of anti-D. I am very glad that you brought up these cases of anti-D in Weak D type 1,2 or 3. It truely is a great opportunity for further discussion. I admit that I can be neglectful when it comes to terminologies (which my mentors will not be happy about). I would really appreciate it if you would take the time to point our my terminology errors since it is something that I am trying to improve on. I am the type of person that constantly need to be nudged when it comes go terminology. (yes, one of those blood bankers!!!)
  14. I wonder if there is anything else other than anti-A in this baby. Does mom has history of antibodies? Does Acid Eluate tested off baby's red cells?
  15. In this reference laboratory, either reference lab or submitting hospital decide whether to test RHD molecular or not. The RHD geno here is not by HEA beadchip but by other in-house methods. For the purpose of simplicity for our transfusion services staff, we translated the RHD results in terms of potential to produce anti-D and code them in as Antigens in our LIS. If RhD negative blood is warranted, we post D* as antibody (communicated to staffs as "give D- but patient has not make anti-D), so that LIS will stop us from issuing D+ units. For patients with weak D type1, type2 or type 3, we used the codes WKD1, WKD2, WKD3 as antigens. For patients with RHD variants that has potential to make anti-D. we used the code WKDV as antigens. We also put in predicted RHD variant in patient's comment (for example RHD*DAR or weakD 4.2.1 etc) Also post D* in antibody profile so LIS will stop the tech from issuing D+ units. For patients with RHD variant in heterozygote expression but also has normal D gene- we do not post anything in antigen or antibody profile. However we free text the predict allele in the patient's comment. For example, "Genomic RHD testing indicates a normal D allele with RHD* DAR1.2. (I suppose posting other terminology. like weakD 4.2.2 instead of allele name will be fine too) Hope it is helpful
  16. that is such a lovely service! AABB just published a checklist for this. https://marketplace.aabb.org/ebusiness/Marketplace/AABB-Checklist-for-Labeling-RBC-Units-with-Historical-Antigen-Typing-Results/ProductDetail/14111716
  17. Here is a very good case with detailed review of serologic work up. I hope the link work. If not, the source is here. Immunohematology. 1997;13(2):58-60. https://p.widencdn.net/b1a7rz/13_2_97
  18. Wow Congratulation. Very well deserved. You have help educate not only your local blood bankers but also from those all around the globe. What an honor!
  19. Hello, I just thought about a question related to gel testing. Would it be fine if neutralized plasma (for example, as part of antibody identification for Chido/Rodger) is tested in gel card? I have always tested neutralized plasma in tube method but always wondered if gel testing would work when I am short of test plasma. I only have to use 25ul of plasma in gel instead of 2 drops (100ul) in tube method. Thank you for useful references and being a great resource here for us, Malcolm!
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