Sadique.Kareem

welcome. your are also one of the lucky persons as i am.there is onething which makes this site learning portal and that is everyone here adds something more to the comments. i feel that every one here thinks, its a moral responsibility to add and share the knowledge. i have never seen any site were there is such a competition in pouring knowledge.

you are right david the discusion was about 2 cell Vs 3 cells but for me the eye catching was tube technology. this is because i am not very senior in this field and started my carier with gel tech only so i thought when we are using gel everybody might also be using gel only. i was just curious to know whether there is any technical significance for tube.

Dear All. I wonder why all of you are talking about tube tech for antibody why not gel cards. here we are using gel cards only. Is there any significance in tube, that i may not be knowing or is it just cost saving.

Thanks rravkin. But the way you mentioned will detect the mixture of IgM and IgG. Where as we are intrested to know the titer of IgG only. the only way for this it seems is that tedious job of using DTT.

We go mid way to be safe. We do antibody screening of all patients samples (EDTA) using gel tech and monospecific anti IgG. To avoid the rare miss of complement binding anitbody we do cross matching by serum from red top tube and polyspecific IgG/C3d.

Thanks david. yes its estableshed fact IgM donot cross placenta but few new borns are seen giving reverse reaction with A1 and B cells. The reason is not clear and I have seen some discusion in our forum ( How Anti A and B are produced) about this issue few months back. I personally came across few new borns 7-10 days of age with these antibodies.

R1R2 yes you are right. Actually i put the question in a wrong way my intention was to know how to detect high titer IgG antibodies in group O mothers.

Thanks Malcolm. could you please clarify what is the exact meaning of high titer antibody in group O mothers and how to detect them.

Hello I want to know how to check for Anti A and B in neonates for PRBC non O group transfusion. It is said to include coombs serum while testing. please correct me if i understood wrong . The way I do is :- 50 ul A1 and B cells in Diamed IgG card + 25 ul of plasma, incubate for 15 min at 37*C and then centrifuge for 10 min. My concern in this is how can i be sure that i am not catching IgM anti A and B that may be present (rare) in some neonates. Thanks.

I have 70 yrs old man whose blood group in our records is B positive. Anti D 4+. Yesterday he was admitted for TKR and needed blood transfusion. While reconfirming the group from fresh sample the reaction in Anti D ( Diamed gel) shows 1+ reaction. Read about antibodies going down by age and also A and B antigen but any one has any referance confirming D antigen weakening due to age. Thanks.

I just read the referance in mollison and now i am scared because in our practice we never cared for the blood type when issuing platelets for new borns. Now if one of my patient has any of these anti A or B ( which i will never know as its not the practrice to do reverse type) and if i give type non specific platelets which usually have few RBCs it may end up in hemolysis of tranfuse RBCs which may be fatal considering the size and volume of new born. TO avoid this minor cross matching would be the only way......

We dont use cord blood due to many problems as mentioned by some of our collegues. but we use venous blood for typing and CxM because it gives the sense that your are working on the patient sample itself. For antibody screenig and identification if needed ofcourse we use mothers sample. But our practice is to issue O Cells irrespective of type of baby ,so we dont do Ant A and B.