dnielsen

The purpose of the PT test is to compare data from different labs. In order for this to work it requires that everyone follows the same procedure, plating the same number of cells. The instructions that come with the kit need to be followed in order to be 'successful'. I run the PT program at STEMCELL Technologies. Problems with making the dilution and plating the appropriate number of cells are commonly the source when a participant is consistently out of sync with the rest of the group. I realize that the procedure we give is likely very different from your standard procedure. The problem is that there is not just one way to set up a CFC assay. We have chosen a method that has been used by some labs for many years. We are always open to feedback, so please feel free to let me know how we can make this program better. I would be happy to talk with any of you about your particular issues. We can go through the calculations together and troubleshoot where things might be going wrong for you. We do have a video on our website and a technical support team that is also ready to help at any time.