Hi everybody I hope you are all well when there is no antibody detected in a patient we can transfuse blood by electronic crossmatch that's mean with complete crossmatch. and if we do complete crossmatch with out doing antibody screening and even the complete crossmatch is compatible this technique is not acceptable because the possibility of producing booster dose for undetected antibodies when we didn't do the antibody screening. the question is if we give the patient unit of blood without complete crossmatch when there is no antibody detected in the antibody screening, is it possible to produce antibody to antigen which is not found in the screening cells or even acute transfusion reaction to antigen which antibody is actually present? Thanks