Mabel, I read on another thread from a couple of people at the reference lab that they were able to get negative antibody screens in tube with LISS. I tried that on one of our patients (we only have 2 so far) that had a positive screen in gel and was sent out for DTT treatment with no antibodies found, and the tube screen was negative. We did not read at 37. I had gotten a pre-treatment specimen and antigen typed it for all anti-sera we carry (Fy, Jk, K, S, C, c, E, e). If you do a LISS screen and it's negative, you don't have to give phenotype-matched products since nothing was destroyed. You don't do that with regular patients, right? I even emailed our reference lab, since technically these patients would be eligible for EXM with no history of an antibody, but I think I'll stick with IS XM.
So, post-treatment screen in gel, if positive, screen in tube, if negative, do IS XM.