We do all DATs in gel. We do baby DATs by washing the cells, spinning to concentrate, taking 10 ul in 1 ml of MTS buffer, adding 50 ul to an MTS IgG card and spinning. We do complement DAT by the same method, using a buffered gel card, 50 ul of 0.8% washed cells. We always run a positive and negative control, since we want to make sure we have added the 25 ul of anti-C3b, C3d to the card. Otherwise everything will be negative!