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Everything posted by Desoki
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that you said, same my explanation
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Yes but I want to continue the story, in those cases, I usually issue units by tube cross match and also observe unit transfusion, no one of these units made any reaction neither acute or delayed and patient usually discharged safely from hospital with good conditions, even with immune-compromised pt. like leukemia or end stage renal disease. So, shall I consider that reaction with gel is nonspecific reaction, resolved by washing during IAT tube method?
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Dear Malcolm, This is just virtual example not true case. Because I can't remember exact case, but by that example I can explain my question, again my question is could dosage effect happen with heterozygous panel cells although reactions with other non-dosage cells were ranges between grades 3-4?
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Yes Malcolm, For example when I did 11 cells panel for that patient, the result was reaction grade 3 with cells number three and five (note that cells No. three and five are homozygous for E antigen), this clear for us that, this patient has anti-E, but also I can't rule out anti-JKa because that possibility of dosage effect on cells number six (heterozygous for JKa and JKb leads to negative reaction), and I have no more further investigation available in my work place as selected cells or others.
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Dear colleagues, Did these cases happen with you, it happened with me 4 times in last three months? Patients' plasma was pan-reactive grade 3 with all tests done by column agglutination techniques (gel cards Biorad company), includes three screening cells, auto-control, 11 panel cells, also cross match with 4 units same blood group were incompatible grade 3 with all units tested by gel cards. When we switched to manual tube saline technique, all tests were negative includes antibody screen, reaction with 11 panel cells, and cross match with same unites that were positive with gel, these tests were confirmed macro and microscopic, and also by Coombs' check cells. What is your interpretation!!!?
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Dear colleagues, Could dosage effect happen with heterozygous panel cells although reactions with other non-dosage cells were ranges between grades 3-4?
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Dear colleagues Anyone have reference about safety to transfuse Rh positive PRBCs to Rh negative patient after fulfilling the following conditions: There is shortage or low stock in Rh negative PRBCs units in your referral blood bank. The Patient in emergency state. The patient is male any age or female after 55 years old. The patient has no anti-D (negative IAT or positive IAT for other cause than anti-D) Thanks a lot...,
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the only problem is presnce of ati A1 (however there is severe doubt about its significant), so I think if these is no anti-A1 no problem to issue A group, but if there is anti-A1 best to issue same subgroup or O. - - - Updated - - - the only problem is presnce of ati A1 (however there is severe doubt about its significant), so I think if these is no anti-A1 no problem to issue A group, but if there is anti-A1 best to issue same subgroup or O.
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Yes its very good idea
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I supposed that mostly clerc. or tech. error, the incidance of that O negative pregnant to produce anti D because she is actual patial D is very rare accorrding to AABB tech 17th edition>
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I suggest use tube panel because it is less sensitive to detect very weak Rh Ab
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According to statement "to avoid dosage effect of heterozygous antigen" use two cells or more of screening cells for patient, but why it is allowed to use one pooled screen cell for donors?
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yes in order to depend computer registered blood groub in issuing plasma or platelet, the blood group should done on two different sample.
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Crossmatch by gel technology only - Is it sufficient ?
Desoki replied to subbusp58's topic in Transfusion Services
Yes, I'm sorry I mean anti A1, but it's mentioned clearly in AABB TM 17th edition anti A1 is clinically insignificant. Thanks -
Crossmatch by gel technology only - Is it sufficient ?
Desoki replied to subbusp58's topic in Transfusion Services
Sure according last standered of AABB if the antibody screen is negative you could issue blood by using one of three; immediate spine cross match, full AHG cross match or electronic cross match, and mostly antibdies interfere with IS cross match are insignificat execpt anti A, anti B or anti AB, also note that anti A2 is insignificant. At my lab we depend on IS cross match if antibody screen was negative, but for us to be more sure if IS is positive we switch to AHG cross match. -
Happy birthday my dear friend Malcolm.
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Reasons for Compatible cross match between A+ and B+ blood
Desoki replied to lyla_n's topic in Transfusion Services
Did you shech QC of your machine and reagent -
i think if you did hb no need to do hct or vise versa
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if Crp postive with high titre it means acute reaction, so i think it's better to deffere utill cure
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I didn't read any thing about upper limit weigth in last TM of AABB or STD
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Dear colleagues, Could you please tell me conditions to discard platelets (after follow instruction for platelets separation and storage) other than bacterial contamination, loss of swirling or lipemia. Is platelet clumping after storage cause for discard or not use platelets? If anyone has any document please provided me. Many thank my friends.
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Dear colleagues, Anyone knows why autoantibody may react with all cells types whatever self or nonself? Thanks for help
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Validity of combs check cells
Desoki replied to Desoki's topic in Immunohematology Reference Laboratories
Sometimes there is shotage of supply -
Shall I consider this anti M is clinically significant ?
Desoki replied to Desoki's topic in Transfusion Services
No Problem take your time, also thanks God that you and your family are fine -
Shall I consider this anti M is clinically significant ?
Desoki replied to Desoki's topic in Transfusion Services
Also Malcolm, you didn't give me your opanion about question of comb's check cell validity?