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April 2024 Challenge

Injun Blood Doc

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  1. Injun Blood Doc
    Echo has had software and camera problems in the past, as you mentioned, with promised fixes in the works (Immucor has been promising these fixes a long time now). Seems Immucor has gotten too big to respond quickly - may be related to turnover of key personnel and management. The Echo camera processes four well images at a time, versus 48 wells for Galileo, so much more of the well is "seen" and processed on the Echo. Maybe the extra detail and information is causing problems on the Echo - I'm not an engineer or optics expert and am only speculating.
  2. Injun Blood Doc
    This usually occurs when the lab technician brings reagents out of the refrigerator and either shakes or swirls red cell reagents to re-suspend them, and in the process, causes the antisera reagents to foam (high protein content permits foaming to last for a while). When the probe enters the foamy reagent, the level sense is fooled by the foaming and thinks reagent has been picked up. The proper technique is to bring reagents out of the refrigerator, make sure a stir ball is in all red cell reagents, and place on the instrument without mixing. The instrument mixes red cell reagents automatically if a stir ball is present. No foaming should occur.
  3. Injun Blood Doc
    There have been reports of positive Capture-R Ready Screen and Read-ID reactions (positive in all wells) due to antibody binding to cryptic RBC membrane antigens exposed in the RBC membrane drying process and to components in the Capture-LISS dye component) reagent. What to IAT in tube or gel indicate? These Capture problems do not occur in the other test procedures, and while rare, should be considered a limitation due to the time and effeort needed to resolve the problem. Were all screen/ID test wells the same reaction strength, and how strong were the reactions?
  4. Injun Blood Doc
    Jeff, Any chance the anti-K has a major IgM component. Capture does not detect IgM, wherease the gel does.
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