Thanks a lot Rashmi for ur reply. We do not use our phenotype reagents for a months and we do not leave them on board for a long period. We had this problem at the begining when we introduced Galileo. Immucor support instruct us to keep the reagnt back in the fridge when they are not in use. We did not see the growth after that, but they are back again even though we follow the same instructions. There must be some thing else, don't you think?