Our transfusion reaction workup standard has been to do a clerical check, dat, and visualization of serum or plasma for hemolysis. If these are negative, then the workup is signed out as "no evidence of hemolytic transfusion reaction" and no further workup is done unless some unusual circumstance or finding is present to suggest something else be done. It is fortunate, I think that it is rare that any of the first-tier studies are positive and the workup is virtually all the time limited to the first tier studies. One observer at our laboratory has voiced an opinion that in virtually every transfusion reaction workup, this limited workup is not adequate, and that every workup should include pre and post total and direct bilirubin, LDH, and haptoglobin as a minimum. This observer feels that the current standard of care requires these tests be added to the first-tier battery of tests. Could members of this forum comment on what your standard first-tier testing consists of, and how often you find that you add more tests when the first-tier tests are negative? Thanks for your input. and Happy New Year to all on the Blood Bank Talk forum! Keath Wade