We noticed that when using the Immucor complement cells with the Ortho anti-C3d, the results were non-existent even after 5-10 minutes incubation at room temperature. At first I thought the problem was with the complement cells, but after ordering Immucor anti-C3d, we noticed the reactions were stronger. After evaluating the Ortho and Immucor anti-C3d we decided to use the Immucor product. I also noticed that the Ortho product was anti-C3d, while the Immucor antisera was a combination of anti-C3d,C3b. Ortho offers an anti-C3d,C3b product, but I never evaluated this product. I figured; why not use Immucor antisera and controlling it with Immucor C3d control