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April 2024 Challenge

Yanxia

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Everything posted by Yanxia

  1. Yanxia
    In China we will do 4 ,22 and 37 degree C incubation with and without oxygen respectively.
  2. Yanxia
    I have several question about this topic. I wish I can get help at here. Thanks in advance！ How to quantitate the anti-D antibody? Does it need D positive RBC? And how to eliminate the antigen intensity difference? Some paper says testing the same sample in different laboratory will have a big differentia,so we prefer to use a new method . Does the notation of"iu/ml " can resolve the differentia between laboratory?
  3. Yanxia
    Thank you.
  4. Yanxia
    Thank you ,Donna. I can remenber when I send the first post I am very nervous. I don't know whether I will be accepted . But I have courage, although it dose not always take good luck to me, this time it dose.
  5. Yanxia
    And use the antigens negative (negative to all the antibodies in the serum )to do the test, if it can react with the cell, then the antibody is not Matuhasi Ogata Phenomenon , is mimicking Ab or nonspecific adsorb.
  6. Yanxia
    Nice to meet you all! I come here not very long, but I think I love here and love all the people who have communicated with me and who will know me. Shily is my name on net .
  7. Yanxia
    I don't think mimicking Ab is the same as Matuhasi Ogata Phenomenon . Becasuse sometimes we can test the DAT positive just because the mimicking Ab lonely on the auto-RBC,and the antigen is not present on auto-RBC. And I have search an assay about Matuhasi Ogata Phenomenon, its address is below http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1423039&blobtype=pdf
  8. Yanxia
    Sorry, I can't understand what is the meaning of Matuhasi Ogata Phenomenon in Chinese. But in China we have met Mimicking Antibody present lonely without another autoantibody. And some Mimicking Antibody's corresponding antigen present on the RBC,some not. All Mimicking Antibodies can be adsorbed by antigen positive and negative RBC.
  9. Yanxia
    Mabel Adams, I have several questions:why we can't use monoclonal anti-A in this test? And I don't understand the meaning of a positive control . I think you can use normal saline instead of anti-A as negative control.
  10. Yanxia
    Mabel Adams, I want to say you are so lucky. Your husband is Le(a-b-), perhaps he is a secretor, there is a little trouble. And Le(a-b-) secrete person will have more ABH substance in their plasma/serum than other person. I think test his saliva substance before do dilution is better.
  11. Yanxia
    Thanks Rcurrie and Marilynm. I guess mybe because the A substance in the A2 plasma reacted with the lectin. Marilynm says "when you mix monoclonal antibodies with human antibodies of the same specificity (Rh D for instance), you sometimes get a weakening of the human antibody" I am very interesting in it. May I trouble you to explain it ?
  12. Yanxia
    Hello,Marilyn. I come from China.I come here to learn bloodtransfusion medicine and English.I am glad to see you serologist at here. I have a question,if you have time,please help me:why anti-A1 lectin added to A2 person's serum will become weaker . Can use a nonsecretor's serum resolve this question? Thank you!
  13. Yanxia
    I am very happy to meet all of you . We use monoclonal anti-A reagent and it will give a 2+mf reaction with A3 . I want to know how strong will it react use anti-A monoclonal antibody produced in USA.
  14. Yanxia
    In China, we don't determine the A2,Aint subgroup routinely except A2 person have deduced anti-A1. A3 and other A subgroup will give a weak reaction when tested with anti-A,so we will find the reason. A3 is especial because some disease will weaken the patient's antigen and give a mf reaction like A3. Because English is not my mother language, my post will give some difficult to understand .Sorry!
  15. Yanxia
    And part of Le(a-b-) are non secretors.
  16. Yanxia
    Thank you Rcurrie. In China A3 with anti-A1 is not rare. I remember the A3 type have different transferase base, some can test the cell A transferase and some cann't. I just wonder if this will like partial D and weak D, the former can produce anti-D.
  17. Yanxia
    My question is can we use A type blood to A3 person , of course O type is safer. If which type we choose depends on the reverse type(have/not have anti-A1)?But the anti-A1 is cold reactive antibody and will not react at 37degree C. The genotype of A3 are different, dose this play a role for deciding which type to use?
  18. Yanxia
    I have an interesting qustion: Is every A2or A2B person with anti-A1 nonsecretor?
  19. Yanxia
    And you can use the suspect blood pack RBC to test the serum and the eluate to find the antibody to low frequency antigen.
  20. Yanxia
    Thank you Labgirl153. I am not an Indian. I come here just because I love bloodtransfusion medicine and want to learn more about it. This forum will not refuse a learner and a participant , ah? Thank you again for your post.
  21. Yanxia
    Is the antibody screen positive or negative? Mybe it is because not wash clear.
  22. Yanxia
    Sorry, my meaning is the HLA and HNA antibody screening and crossmatch used to test the incompatibility of HLA and HNA between the donor and recipient. Sometimes to use a more sensitive method will find something new, mybe can open a window of resolve for this difficult problem. I have noted that the TRALI will have a lower blood pressure than normal and the TACO's blood pressure is higher than normal. So to distinguish them is not a difficult work.
  23. Yanxia
    I have read an article which said a patient with TRALI had a positive antibody screening but negative cross-match with the donor blood(HLA and HNA).Then they use the flow-cytometry method can find the discompatiblility.
  24. Yanxia
    We don't use anti-A1 except the appearence of discrepancy in the blood group test, so some A2 may be reported as A. But when we have a negetive result with anti-A1 and a reaction which is stronger than 2+ with anti-A, we will report it as A2.
  25. Yanxia
    I will use the method in which we find the the previous antibody and IS to do the crossmatch.
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