Abdulhameed Al-Attas

Yes, I vote for one blood type/admission.

Our Hospital consider CMV seronegative and leukocyte reduced blood products equivalent and would consider them equivalent for all patients. This includes low birth weight neonates. We use these components interchangeably for all transfusion recipients including neonates.

Our policy is that our neonatologist is the one who determines which neonate receives irradiated PRBC's,But ususlly Pre-terms are the ones. Once a unit is irradiated for a neonate,it must be used with in 24 hours.

I am a student of Malcolm,but I can help; just double click the small graph and it will turn big enough to be useful.

Thanks Malcolm,for the great explanation as usual.

Titre: The reciprocal of the highest serum dilution that causes macroscopic agglutination when serial dilutions of an antibody are tested against selected red cells. Applications: Prenatal testing, IdentificatioofHTLA, Complex antibody identification, Differentiation of pathological and harmless autoanti- I and Procurement of antisera Quality assurance of reagents. Limitations: Titrations are only semiquantitative estimates of antibody reactivity due to several variables that affect their performance. Three main variables are the technologist, the red cells, and the method. Ways to Minimize Variables: technologist: experienced with proven technique red cells: ideally when titres of samples are to be compared, use fresh red cells (antigens deteriorate on storage) from the same donor (same number of antigenic sites present). If this is not possible, use commercial red cells of the same apparent genotype. method: when sequential samples are examined for change in titre, store samples frozen and run new samples in parallel with the immediately preceding sample. This is the most practical way to control that an increase in titre is real.Prozone Phenomenon: This may cause reactions to be weaker in the first tubes than in higher dilutions and is believed to be caused by an antibody excess in which all antigenic sites are sensitized with antibody leaving none free to form cross-links Significant Difference in Titres: When comparative studies are done, such as in prenatal testing, a difference in titre of at least 2 tubes is required to be considered a significant difference. For example, if the titre changes from 32 to 64, this is not considered to be significant (difference of only 1 tube); however, a change from 32 to 128 would be significant (2 tube difference). These are the important points to know about AntibodyTitration, in case you may need more, let me know.

We are very saddened to hear to your loss and would like to express our sincere condolences to you and family. Remember that we ( in PathLab Talk ) love and care about you.Our thoughts and prayers are with you and family.

I am curious to know because to x-match 50 units through second stage enzyme is really time consuming and is NOT required as Eagle Eye, has stated above. Drwajiha,could you please explain how you enzyme X-match?

What about serum specimen with AHG monospecific IgG, Can I still have hemolysis as a positive reaction; because I use serum (source of complement)? Or something else is needed along with the serum?

Yes, I agree, a huge loss to the Immunohematology and Transfusion Medicine Community.May he rest in peace,

Welcome to all the new members! It is really nice to have you in this wonderful site.

The DAT strength does not correlate with the severity of the hemolysis. In Rh D hemolytic disease, the DAT may be strongly positive without clinical signs of disease; whereas in ABO hemolytic disease,clinical features may exist with only a weak or negative DAT. In the transfusion reaction as Malcolm, mentioned; you may have a negative DAT if all the transfused donor cells (that are carring the corresponding antigen) are removed from the system.

HI David, The correct spelling is : Matuhasi-Ogata phenomenon.

I agree with David Saikin.

Yes, I completely agree with John, the only change I have seen is about the contact individual that makes the MTP activation is either OB physician or Perinatologist instead of a Surgeon or Intensivist.

Annually

Additional testing of the red blood cells with Anti-A,B reagent facilitates the recognition of certain weak subgroups and is sometimes used as further confirmation of the reactions obtained with Anti-A and Anti-B reagents.

Yes, you are right.

Done

Number 2 for sure.

Yes, Malcolm, you are absolutly right But we in Third countries use ONLY serology Techniques, Molecular is still a dream for us.

Our current policy is that Hemoglobulin S Negative Red Cells will be provided for IUT,regular top-up transfusion and neonatal exchange transfusion. Another thing you can NOT dignose a neonate for a Sickle because the hemoglobin they have is HbF at that age you have to wait untill 7 months to do that.

I completely agree with Anna and John. Yes, aslong as the previous work is accessable,there is a comment in the system that Alerts and the patient has NOT had any BM transplant or Massive transfusion of Group O for non Group O patient,Remember there'll be NO change of Blood Group. So why re-investigate?

Do not exceed four hours when transfusing a unit of red blood cells. If blood must be transfused very slowly, request that the packed cells be ordered as a split unit. Red blood cells left unrefrigerated longer than 30 minutes (temperature exceeds 10° C) must be transfused to the patient within four hours from the time of issue. These units cannot be returned to the Transfusion Service for reissue to another patient. .

Thanks,Anna,for all of that great information.