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Thank you for welcoming me. I ask a question for you about cold autoantibodies. I uploaded this question in the immunohematology reference laboratories section. If you give me some advice, it will be my honor.

Hello, fellow blood bankers. Question for antibody identification of a patient with a previous history of transfusion! What do you make of these results? and What are you going to do next? We performed gel testing using Bio-Rad Liss/Coombs card and got some positive results in IAT. Next, we performed tube testing with Ortho ID cells. We found strong reactivity at room temperature but some reactivity were present in IAT. Autologous control is very weakly reactive at room temperature (IgG and C3d DAT is negative). Do you think some reactivity in IAT (tube and gel) results from cold autoantibodies? Or some IgG alloantibodies coexist with the cold autoantibodies? What do you make of these results? and what are you going to do next? We have encountered such problems often. We really need your help. Thank you.