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SbbPerson

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  1. Like
    SbbPerson reacted to Ward_X in Ortho Gel combo workstation   
    My old lab used to do something similar! Just a dummy slide with the probe wedged in the well and putty sealing around the opening. 
    For those wondering if a thermometer will fit -- there are some that do, and the wiring threads out under the cover using a small notch 
  2. Like
    SbbPerson reacted to Kelly Guenthner in Ortho Gel combo workstation   
    We're still rockin' this original setup on our single combined Ortho Workstation.  It's trash, but it works.  
    If/when it's time to replace our 2 old separate setups, we will try to source an alternative.

  3. Like
    SbbPerson reacted to REN_NH in Ortho Gel combo workstation   
    We document that the light is indeed green, indicating that the factory set temperature is OK, on our Daily Temperatures and Checks form as a check mark each day for the "MTS Incubator Status Light Green?".
    We also check the RPM's daily and record on the same form.
  4. Like
    SbbPerson reacted to psykobillys in BloodBankTalk: QC of multiple reagent racks   
    I just answered this question.

    My Score PASS  
  5. Like
  6. Like
    SbbPerson reacted to John C. Staley in Timeline of repeating the antibody panel for a historical patient   
    Just a thought but you may want to check the AABB Standards instead of the Technical Manual.  Your question is more of a standards question than a technical one.  Since I no longer have access to the Standards this is the best I can do.

     
  7. Thanks
    SbbPerson got a reaction from John C. Staley in Timeline of repeating the antibody panel for a historical patient   
    5.14.4   A new sample shall be obtained from the patient within 3 days prior to transfusion in the following situations:
    If the patient has been transfused in the preceding 3 months with blood or a blood component containing allogeneic red cells. If the patient has been pregnant within the preceding 3 months. If the history is uncertain or unavailable. Day 0 is the day of draw. 5.14.5   In patients with a history of previously identified antibodies, testing shall be capable of detecting and identifying the presence of newly formed clinically significant antibodies. Standard 5.14.3.1 applies.
     
    5.14.3.1 When antibodies are detected, additional testing shall be performed to identify antibodies of clinical significance.
     
    Source: AABB Standards for Blood Banks and Transfusion Services, 33rd Edition, effective April 1, 2022 (Published: 12/21/2023 )
  8. Thanks
    SbbPerson got a reaction from Cliff in Paypal   
    Thank you Cliff! Sorry, I didn't see this reply sooner. Thanks again
  9. Like
    SbbPerson reacted to Cliff in Paypal   
    That's odd. 
    I was under the impression (possibly false) that PayPal was only required for me.
    Anyhow, I just added Stripe payment processing.  When you get to the checkout, you can choose Stripe or PayPal.
    I appreciate your support!
  10. Like
    SbbPerson reacted to John C. Staley in Crossmatching using automation   
    I'm not sure if this is still the case but the IS step was intended to confirm ABO compatibility.   

  11. Like
    SbbPerson got a reaction from Malcolm Needs in BloodBankTalk: Correct Blood Bank Nomenclature   
    I just answered this question.

    My Score PASS  
  12. Hugs
    SbbPerson got a reaction from Cliff in HemeLabTalk: Anemias and Myeloid Malignancies   
    In my opinion, this one was hard. I had to do research
  13. Like
    SbbPerson reacted to Tessa in HemeLabTalk: Anemias and Myeloid Malignancies   
    I just answered this question.

    My Score PASS  
  14. Like
    SbbPerson reacted to Texas Tea TMC in IS XM Positive-Next Step?   
    3 common interference possibilities
    Rouleaux - look at the reaction under scope, see if you see the coin stack appearance. Resolve IS interference with saline replacement
    Nonspecific cold agglutinin - perform mini cold screen   - one group O big I+ adult cell (screen cell works fine), one group O i cell (cord blood), and autocontrol. 2 drops patient plasma to each of those cells.  Read at IS, RT inc and 4C inc. If autocontrol and adult cell are positive, and reactions increase in strength with refrigeration, its usually presumptive nonspecific cold agglutinin. Resolve with prewarming the plasma to reduce interference at IS phase. May not work if cold agg is strong.
    Cold preferring IgM antibody like an Anti-M or P that doesnt show in gel but may interfere in tube if the crossmatched unit is antigen positive.  Run a 3 cell tube screen with IS phase and a RT phase to get clean strengths to id the antibody. Your hospital policy should specify whether you need to antigen type units to get real crossmatch compatible, or just crossmatch untyped units until you get a IS XM compatible one. Most hospitals consider the common suspects (M, P, Le) not clinically significant if they are only showing at IS, but if you work at a place that requires IS XM on all rbc orders, and they want it "compatible" you might be stuck antigen typing.
    And lastly always a chance the unit or patient ABO result is incorrect, repeat the ABO typing on patient sample and unit.  
      
  15. Like
    SbbPerson reacted to donellda in A friendly competition   
    My Fitbit Blaze arrived today. Now to figure it out. Thank you again!
  16. Like
    SbbPerson reacted to donellda in A friendly competition   
    Thank you 
  17. Like
    SbbPerson reacted to donellda in BloodBankTalk: Correct Blood Bank Nomenclature   
    Ha ha! Good thing I retired then
  18. Like
    SbbPerson reacted to Malcolm Needs in Anti D level in 2nd pregnancy   
    The simple answer is gagpinks, but this is the answer I have just received from my friend at the IBGRL (who shall remain anonymous for now).

    The question I put was as follows:

    "Sorry to bother you yet again, but I have had a query from a friend. I think I know the answer, but I wanted to check with an expert. If a pregnant lady has an allo-anti-D, can this affect cffDNA harvesting from the mother's circulation? I don't think it does unless the anti-D knocks out all of the foetal red cells. Best wishes from this bloody nuisance, Malcolm"
    Answer below.

    "Hi, that's right, anti-D makes no difference to the cffDNA test. The two biggest problems are false negatives due to insufficient RHD gene in the test sample and mums with a RHD gene (despite pheno typing as D-) leading to strong positive results. Take it easy."

    As I said, the friend will remain anonymous for now, but, suffice it to say, he/she is one of the people who do the test, so I think the answer can be trusted!
     
  19. Haha
    SbbPerson reacted to Sherif Abd El Monem in Study With Me : Introduction to Blood Transfusion 1   
    🩸 Exciting News! 📚 Discover the fascinating world of blood transfusion by reading the book titled "Introduction to Blood Transfusion: From Donor to Recipient," published by the International Society of Blood Transfusion (ISBT). 🌍 This book will be presented in a simple question-and-answer format for easy understanding.
    https://immunohematologymadeeasy.com/study-with-me-introduction-to-blood-transfusion-1/
    Follow this in comments.
  20. Like
    SbbPerson reacted to gagpinks in Anti D level in 2nd pregnancy   
    Hello 
    Patient has developed antiD in first pregnancy at around 32 weeks and her quantification level was 1.0 IU/ml.
    In 2nd pregnancy her booking blood at (12 weeks) antibody screening was negative.  At 15 weeks sample sent for fetal genotype (FDS). On this Report received inconclusive due to all Anti D.
    Because patients was on file for historical antibody therefore sample sent for quantification  in 2nd pregnancy and Report received antibody not quantified since it reacted weakly in enzyme IAT only. 
    My understanding standing is if patients once developed Allo antiD her titre level does not go down. Why was her antibody screen was negative in 2nd pregnancy at 12 weeks?
  21. Like
    SbbPerson reacted to AMcCord in Anti-CD36 (anti-GPIV) Frequently Interferes With Routine Red Blood Cell Antibody Testing in Patients of African Descent-References?   
    I didn't find much either, but from what I did find it looks like it could be another interesting blood banker's problem since CD36 is definitely found on RBCs. And it sure looks like it may be utilized with immunotherapy for multiple kinds of cancer treatment to make it more effective. Or for FNAIT.
    https://pubmed.ncbi.nlm.nih.gov/1382721/
    The link below is interesting - not sure how it would relate. 
    https://pubmed.ncbi.nlm.nih.gov/8623134/
    And then there are these tidbits:
    https://pubmed.ncbi.nlm.nih.gov/34041523/
    https://www.ahajournals.org/doi/full/10.1161/01.atv.16.7.883
    If patients with African descent are more likely to develop anti-CD36 due to CD36 deficiency, are their red cell ABO types (or other antigen types) going to be affected by that antibody (which would be an autoantibody)??? 
    I'm with Mabel - anyone out there know anything about using anti-CD36 for cancer treatment or FNAIT treatment?
  22. Like
    SbbPerson reacted to Yanxia in Cord Blood testing on gel   
    Sorry,I can't find the English version.The  underlined sentence means the cord blood cells needed to be washed at least once before testing.
  23. Like
    SbbPerson reacted to Mabel Adams in Cord Blood testing on gel   
    I only wish I could know another language anywhere near as well as Yanxia knows English!  She has always impressed me with her blood bank knowledge as well.
  24. Haha
    SbbPerson got a reaction from Yanxia in Cord Blood testing on gel   
    My Chinese is a bit rusty, but if I am not mistaken, the underlined sentence states that we need to wash cord blood at least once before testing.  Sorry, I am just kidding, I don't know any Chinese, I just read Yan's explanation. 
  25. Like
    SbbPerson reacted to Yanxia in Cord Blood testing on gel   
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