1. In the book Judd's Methods in Immunohematology, auto adsorption is not feasible if pt transfused in 120 days. but in AABB tech manual method, the cut off is 3 months, i.e., 90 days. Would 90 days acceptable for auto adsorption? or have to stick to 120 days? any input?
2. AABB method implied that the typing on patient's reticulocytes by harvesting autologous red cells should be exercised with caution for interpretation of the E, e, c, Fya, Jka, and Ge antigens. How can be confident to the typing results for those antigens just mentioned? If no confidence, this method is not valuable to type the pt recently transfused. The reason I asked is that I eagerly to find ways to use pt typing information to assist allo adsorption.
3. I can use genotyping information, but it will take 2-3 days to get results and we may not wait so long in some cases.