Hi guys,
I really need some help with setting up the DTT for DARA patients protocol at my hospital.
1. Which reagent do you use? We prefer the prepared 0.2 M DTT. Which manufacturer do you buy from?
2. Could I get a peek at your SOP?
3. Do you use single-use DTT preparations to repeat the antibody screen? Do you instead prepare a bunch each month and preserve in Alsever's solution?
4. Is it ok, to just repeat only the antibody screen and not do the screen and panel?
5. Any other tips/advice are MUCH appreciated! :)
Sincerely,
Gloria N.