radwan1411

For us blood grouping regarding the donor unit is done 1- ( Segment ) after collecting upon recieving at separation area. 2- Tube + Segment ( donor screening ) 3- Segment with the Cross matching as final typings.

Or blood bank Module BestCare does it like this,, The physician will place an order , the order request goes to something called ( Transfusion order screen ) and at the same time system issue a panel of tests for that patient this panel has ( blood group + Antibody Screen + AC+ 2nd sample + X-Match ) once the 1st sample is arrived all the tests will be done to it except for XM & 2ND sample will be left empty ) once the nurse call for preparation or informing that this patient OR is tomorrow the staff always check the result Screen if the X-M TEST + 2ND is empty they as for a second sample once that sample arrive the result of XM + 2ND is updated with the blood Group of the patient ) 2nd sample is like a test placed with the panel and is there only as an evidence of recieving and for tracking and u can monitor time with it and who done like every other test and has no charge code

DAT : 4+ BTW: Patient was transferred to another facility, after performing 70+ (A,O) units all Incompatible 1+ and more, even same phenotype also as well Incompatible.

- Patient has Transfusion history type to type units he received. - DAT 4+ - we use LISS and the only medium available. Physician diagnosis : Hemolytic Anaemia.

Hey guys.. I just had this case today in blood bank, and i couldn't id the Antibody in this patient serum. All i know is this : - the patient is A pos , the cassette control is +positive - AC pos 4+ - gives in Untreated id panel 4+ in some cells and w+ in others as shown in the picture, and when we used the Ficin Treated panel all cells from 1 to 11 all 4+. - at first i thought about Anti-D but this patient is already Rh pos. And i reached a dead end. - BTW he gives w+ incompatibility with all A pos units. Just ignore the blue highlight, one of the staff but im not convinced its one of those.

for us here,, for the antibody ID we use CCC + Anti-D diluted 1:256 at least for every lot that come in

you are welcome ,, Indirect Coombs Test or Antibody screening

we prepare our own QC for the vision, and it's working perfectly.. because sometimes the ortho confidence is not coming regularly and delay in the shipment occur. our QC goes like this : your will need a : - patient sample in EDTA with blood group of O and call it QC1 - patient sample in EDTA with blood group of B and call it QC2 - patient sample in EDTA with blood group of A and call it QC3 - patient sample in EDTA with blood group of AB and call it QC4 Regradless of the Rh, but one of them must be Rh negative and Du must be performed for detection. ---------------------------------------------------------- for ICT : the rule is to check every Panel 1 to 3 if they are working properly : so it goes like this : - place an anti-D in plan tube and mark it as QC5 = mostly will give you panel 1 and 2 ,,, so we need one addition antibody to test panel 3 - place 5ml a different antibody in plan tube as long as it's testing panel 3 this will complete the reaction of the ICT panel 1.2.3 and mark this and call it QC6 --------------------------------------------------------- For phenotyping - use a patient sample and use it as a Rh phenotype sample EDTA and if it could be K+ ag that would be better call it QC7 ---------------------------------------------------------

hi ksmith ,, regarding the thermo cw3 here in our section we have it for 6 month, we use it around 10 times/ day no problem at all unlike cw2 with the plastic rotor inside lots of problems with that one, id say 6 months are not enough to judge but it's doing well up to this moment.

Discard the unit, to be on the safe side even if it's rare.if the doctor insists on transfusing it, do a quick bacterial detection kit.. and make sure that doc sign an emergency relasse form or any of your hospital form that indicate that he knows the risks

Like your lab we do it manually the ebtering of result into the hospital information system, there were some mistakes in the past that has been resolved with second sample system, the rule we are using is two sample drawn by two sifferent nurses witg 30 mintues gap or more .. we are doing this until we interface the vision in the future

Thats was in our laboratory policy as an indicator of quality control, for cap and aabb checklists, if there is a discripency as above there should be a corrective action after reaching to the result of the discripency

Yes correct , 10 sample from each abo and rh, 5 negative ICT and 5 positive , 5 negative DCT and 5 positive , the both methods results must agree

The ICT was done on the same donors twice not different donors and we do this two methods results comparison in our lab and compare the results every 6 months and we picked up on these donor this time the first donor case was resolved it was a cold antibody, I did a cold ICT using ortho surgiscreen on Reverse diluent cassette and patient sample with bliss no 37 incubation just room temprature incubtion and i was positive still resolving the second donor

hello, guys i'm having an issue with an antibody screening results of the donors i had an issue with couple of donors the first one he's an O+ , on the gel it gave negative ICT, but in the tube method it gave a positive P3 , identification is negative on tube method second donor was positive on the gel method, he's an A- , and the tube ICT was negative , tube identification was negative bio rad reagent used in the tube method and ortho diagnostics in the gel what's the possible causes of these results thanks