Hello, fellow blood bankers.
I got a question about adsorption/elution studies for weak antigen detection.
Literature describes in detail how to detect weak A and B antigens or weak D antigens using this method.
However, literature describes little regarding other than these weak antigens.
For example, when the Kell null phenotype is found, we need to confirm the absence of weak K and k antigens using adsorption/elution technique.
Imagine that we have only anti-K monoclonal IgM and anti-k monoclonal IgG. How can I perform adsorption/elution studies for K and k antigens?
My guess is that for anti-K monoclonal IgM, I can use room temperature incubation, heat elution, and immediate spin test with K+reagent RBCs, while
for anti-k monoclonal IgG, I can use 37C incubation, acid elution, and IAT with k+ reagent RBCs.
Do you have any opinion about this or can share your SOP with me?
I look forward to your help.