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I suggest to my techs during training that it may be prudent to test the last wash before preparing the eluate - especially when additional red cells are not available. I'm sorry to report that I have seen several elutions where the last wash never became negative..... usually with cord cells where the mom had a VERY strong antibody - even changing tubes with every wash. I'm sure that if I had had the patience and persistence, it might have become negative but I gave up at 10 washes.

In my opinion, you can run an antibody screen on the last wash instead of a full panel. Of course if the screen is positive, you'd want to run a full panel. I have never known the last wash screen to be positive.