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In my experience with HTLA like antibodies you usually have a feeling to go that route by the way they shake off, they are generally weaker reactions and sometimes hard to reproduce. As Malcom has stated, I have heard strong Ch/Rg antibodies that need to be diluted out to neutralize. I personally like to do a titer, and I find that antibodies to Ch/Rg usually like gel. But that's when they place nice... Hope that was helpful