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Popular Content

Showing content with the highest reputation on 03/17/2021 in all areas

  1. Hi! I do not know which gel card supplier you are using, but the one I “know” use 50ul of A1 and B cells with 25ul of plasma and an incubation at 37°C for 15 minutes. And all of this in an AHG gel card of course… having previously checked as well there is no additional antibody that could interfere (result from mother if available/antibody screening result). Hope it helps.
    2 points
  2. Thank you Malcolm. Our prenatal titer procedure calls for reading microscopic solely for the purpose of scoring, but I guess there isn't an associated score . Looks like the procedure should be updated
    1 point
  3. Macroscopic every time.
    1 point
  4. Add some Dextran to a plasma sample, it should do the trick and will save you a lot of time and effort freezing and retaining samples etc.
    1 point
  5. When we first started using Gel, my techs would point out the shadows or 'jumpies' as they called them. I'd suggest 'Run the screen again using maxtime.' If they still saw the shadows, I'd just let them run a panel (maxtime) and go crazy with the results. After a while we all learned to ignore those reactions. Keep in mind, there are always a certain percent of any given cell population (especially stored reagent cells) that are just not going to make it smoothly to the very bottom solely because of steric hindrance (broken, aged, crenated, tagged for destruction, etc.).
    1 point
  6. If it was used as part of an antibody workup, I think AABB says indefinitely.
    1 point
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