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Yes, but DVI donors need to be typed as D+. Donors are not patients.

Hope this works for you John, but you are correct; you were not missing much!

When I got to bed last night, I suddenly realised that I may have missed out a fairly obvious cause, as I had not taken into account the fact that the patients were all pregnant. I just wonder if these patients have all made anti-Lea and anti-Leb, as it is not unusual for the Lewis antigens to "disappear" in pregnancy, and quite often they transiently make Lewis antibodies. If none of the cells you are using are themselves Le(a-b-), then this antibody mixture (actually, it isn't a mixture, but anti-Lea+b) can look like an antibody directed against a high prevalence antigen. If you cannot obtain sufficient Le(a-b-) red cells to test this theory (and it is only a theory), you could try to inhibit the antibody with saliva from an Le(a-b+) individual (but don't forget to control this by diluting another aliquot of the plasma with saline). The attached may be of interest/use. The Lewis Blood Group System and Secretor Status.docx