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    Malcolm Needs

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    AB123

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  4. Cliff

    Cliff

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Showing content with the highest reputation on 10/04/2019 in all areas

  1. It could be that the cells are coated but not sufficient to get a positive result. However when you perform the eluate on a large enough number of RBC'c the amount of IgG removed from these red cell's is then enough to coat and cause a positive reaction on the screening cells. Also the cells from the DAT will be heterozygous cells, hence you will get a stronger reaction from screening cells that are homozygous if sufficient antibody is eluted to coat them.
    3 points
  2. One thing I would say is that the baby should be treated on clinical symptoms, rather than on laboratory results, particularly when they are so weak that you have to do all this testing to show an abnormality in the Blood Bank. A slight rise in bilirubin is normal in a newborn baby. This situation is very similar to the difference between a haemolytic transfusion reaction, where, for example, there is a positive DAT, antibody can be eluted and there is a SIGNIFICANT rise in bilirubin and a SIGNIFICANT drop in Hb, and a serological transfusion reaction, where there may, or may not be, be a positive DAT, antibody may or may not be eluted from the red cells, a new antibody specificity may be detected in the plasma, but there is NO SIGNIFICANT rise in bilirubin and NO SIGNIFICANT drop in Hb. Your cases remind me strangely of the latter.
    3 points
  3. Bb_in_the_rain

    Picky anti-C?

    Maybe if you have ficin or papain, you can treat the C+ cells that were originally negative to see if your strange "anti-C" pops up? If the patient is an African American, I would consider the presence of variant RHCE gene.
    1 point
  4. JAT-C Got to the bottom of it with one of them, it was Anti-c antibody positive and was cold reactive with the A cells. Switched for some c- cells and now clear.
    1 point
  5. Malcolm Needs

    DVI

    Partial DVI is the single most common partial D found in the White populations, having a phenotype frequency of between 0.02 and 0.05% in these populations. Have a look in Daniels G. Human Blood Groups. 3rd edition, 2013, Wiley-Blackwell and/or Klein HG, Anstee DJ. Mollison’s Blood Transfusion in Clinical Medicine. 12th edition, 2014, Wiley-Blackwell. You will find several references in either or both of those books. Alternatively, look for http://www.rhesusbase.info/ in your search engine (which is a superb site). It is only really necessary for the "donor side" to look for individuals with Partial DVI. There really is minimal evidence (even that is stretching it) that foetuses/babies who are Partial DVI can cause their D Negative mother to produce an anti-D, but it would be almost impossible to put that particular genie back in the bottle! That having been said, in my experience, the moment that people find that the baby is a Partial DVI, they shoot the mother full of anti-D immunoglobulin, without ever testing the mother to find out if she is also a Partial DVI. One day, a virus, unknown at present, will be passed on in the anti-D immunoglobulin, and it will be proved that it need not have been given in the first place, and then, all Hell will be let loose.
    1 point
  6. Cliff

    To Assess or Not

    I was new to my compliance officer job. I was hoping to learn more than teach. I assessed one organization that almost made me cry. I asked for anything from section 1 in Standards, they had nothing. I asked for training documents, they gave me beautiful blank ones, but none that were completed for any staff. The whole assessment went like that. I went to lunch and was so overwhelmed with a sense of doom for them, I almost wanted to leave. Most of the places I went were similar. I stopped as it was just too draining on me.
    0 points
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