We don't even perform a Pre-DAT, if the post-DAT is negative. How much you do with a positive DAT depends on the comfort level of the pathologist and the patient population you have. AABB is an outstanding organization -- one that deserves support and membership! No one has done more over the years for the blood banking community (from donor to recipient to Techologists to technology) than they have, and other agencies look to them for guidance in the Transfusion Medicine arena, including the FDA. Many times, though, it short-sightedly comes down to dollars in the budget ... One other thing to remember about the AABB is their 20+ year focus on systems, something that other agencies have only recently (and begrudgingly) adopted. They are a progressive voice in the very conservative lab field, and way ahead of everyone else in their view of quality -- indeed, others are following their lead and playing catch-up! Larry Smrz, MBA, MT(ASCP)SBB, CQA(ASQ) Indianapolis, IN AABB Assessor