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Hi Enamul, This is a known "Limitation" of the Vision Analyzer stated in the Operator's Manual-I've added the statement below. "When a sample is collected from a recently transfused patient, the potential exists for the transfused red cells to concentrate after centrifugation at the bottom of the sample tube below their autologous cells. The probe aspirates from the bottom of the tube where the transfused cells generally concentrate which may lead to an unexpected result." We actually have this statement in our SOP as it can be very confusing! ~Ann in CA

Your reverse cells are probably Rh- and may react with anti c. Using gel will only enhance this reaction since the plasma/cells are in contact for 10+ minutes or more especially if using automation. You probably ran your reverse tube without delay so you did not pick up the anti c. If you incubated your tube reverse B cell at 37 you would probably pick up some reactivity. I have seen this many times especially with anti c.

Yes, I centrifuged in my specific centrifuge just for BB. I have found that our core lab centrifuge does not spin the specimens down very well, so I have to spin again in mine.

Congratulations!

Congratulations, Malcolm! Very well deserved of this recognition.

Very well deserved. Your explanations and library contributions have been very helpful to so many.

Would you call that a limitation of the automat? Personally I would not - any more than saying that sampling from the top of the tube is a limitation of manual sampling. It implies that there is a problem and it lies with the automat - when in fact it's a simple manifestation of a biological property of red cells

Yes, this is a known limitation of automated analyzers in blood bank.

There is no standard that requires a transfusion to be started within a certain time frame from release. the only timing that is critical is that the unit needs to be complete within 4 hours of release.