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Showing content with the highest reputation on 06/15/2018 in all areas

  1. What would be really helpful, and is quite simple, is to treat the plasma with 0.01M DTT (or ZZAP come to that), which will denature IgM molecules by breaking the J-chains. This will show if the antibody is IgM or not. Do not forget that some IgG antibodies can cause agglutination in the "cold" and the "warm", with no potentiating agents being present, so it is worth knowing, from the word "Go", with what you are dealing.
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