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Sounds like a leaky segment. Do you get the bag back so you could investigate? I would document this incident as a safety event.

I will go with what the manufacturer states.

This was a hot topic about 2 yrs ago. The FDA came out and said that storage in blood boxes/coolers is considered storage NOT transport. AABB/CAP might be mollified, I don't think the feds will think twice about a 483. Unless you are putting the product in a BB refrig. Check out posts from Feb, 2016

We are using Mediware's HCLL in the BB. In general, ER will know through field triage what is coming so that they can use the registration system and give us a name/MR before the patient arrives. It takes about 10 minutes to order and print tags for a "initial resuscitation" cooler of 2 O neg RBCs and 2 AB FFPs. We are a level 2 trauma center so it is important that a patient can be registered before they arrive. Like many facilities, we always have at least two AB thawed units of AB plasma on hand along with a 5-pack of plts, in addition to the O neg RBCs. Scott

When I was diluting red cells to 0.8%, I ran QC straight from the bottle for tube (because we always do QC for tube), then repeated with the diluted cells for gel. The QC on the 0.8% dilution is going to cover the entire process, including the dilution step, and the reagents. We made up fresh cells every 24 hours, as required by the manufacturer.

RESt = Rabbit Erythrocyte Stroma - basically stabilized red cell membranes from rabbits. There is absolute no DTT in RESt.

Fair comment John, not fatal septicaemia, just fatal cellulitis! My point was though, that it is just not worth taking the risk.

Just out of curiosity, how has this been working out for you? Any significant problems or negative patient outcomes do to this practice? What caused you to pose this question? Also, how big and/or busy is your facility? Is your testing manual or automated? On the surface I would have to answer your question with a NO but a little more info would help. I've always been a "if it ain't broke..." kind of guy. If your system is simple and works well for you why seek to complicate it.

I always considered antibody identification both art and science with a little magic thrown in for good measure.

Sorry guys but you've got it wrong about the witches. she was a witch if she DID NOT drown - in which case she was burned at the stake. whether she had anti-D or not!

I read on the Internet that if a person sinks in water and drowns, they're proven to be a witch........

I've been told that the concentration of DTT in RESt is different that what is used for treatment of reagent cells. But that sure would have been convenient.