Hi Jermin
I think there are a couple of points here that seem to me to be a bit confused.
1. 'The DiaMed reagents are, but not the NBS reagents. Thanks for that question, as it slipped my mind. ' You won't be using NBS reagents to do your Rh phenotype on the IH1000. All you will be using are the cards, which are what you are using now, and diluent 2, which you are using now.
2. I was planning on getting the reagent cells, centrifuge them to separate from the preservative it is in, then resuspend it in PBS. No, Jermin you really cannot do this. It is not the preservative that is the issue, but the concentration. The samples you put on the analyser need to be packed cells - and you need enough packed cells for the instrument's needle to be able to aspirate the correct amount. If you spin down reagents, even if they are at 3-5%, you will not have enough cells in the pellet; even less so if you them dilute them with PBS - which you should never use with the cards anyway as it can cause trailing.
3. I wish I knew how I could do that. I will need to probably check with the manufacturer's reagent sheet and see what sort of limitations and discrepancies there might be, otherwise it might be beyond me. You are currently using the same cards and the same Diluent to carry out your manual testing as you will be using on the Instrument. Any discrepancies you see will come about because of manipulation error only.
Can I suggest that you contact the local Biorad rep who installed the instruments and ask them to help you with this?