Jump to content

Leaderboard

  1. galvania

    galvania

    Members


    • Points

      2

    • Posts

      885


  2. Malcolm Needs

    Malcolm Needs

    Supporting Members


    • Points

      2

    • Posts

      8,471


  3. exlimey

    exlimey

    Members


    • Points

      2

    • Posts

      377


  4. SMILLER

    SMILLER

    Members - Bounced Email


    • Points

      1

    • Posts

      1,373


Popular Content

Showing content with the highest reputation on 12/20/2017 in all areas

  1. I can understand that. In the UK, if the ever pedantic MHRA see that a test should be incubated at 37oC, they will give you a non-conformance if your incubator is at 37.1oC on the day of the inspection. If, on the other hand, you say in your SOP that the test should be incubated at 37oC, +/- 2oC, they are more than happy (despite the fact that we ALWAYS run positive and negative controls with EVERY test). As it is nearing Christmas, I will not write down my true feelings about the MHRA.
    2 points
  2. Hi Jermin I think there are a couple of points here that seem to me to be a bit confused. 1. 'The DiaMed reagents are, but not the NBS reagents. Thanks for that question, as it slipped my mind. ' You won't be using NBS reagents to do your Rh phenotype on the IH1000. All you will be using are the cards, which are what you are using now, and diluent 2, which you are using now. 2. I was planning on getting the reagent cells, centrifuge them to separate from the preservative it is in, then resuspend it in PBS. No, Jermin you really cannot do this. It is not the preservative that is the issue, but the concentration. The samples you put on the analyser need to be packed cells - and you need enough packed cells for the instrument's needle to be able to aspirate the correct amount. If you spin down reagents, even if they are at 3-5%, you will not have enough cells in the pellet; even less so if you them dilute them with PBS - which you should never use with the cards anyway as it can cause trailing. 3. I wish I knew how I could do that. I will need to probably check with the manufacturer's reagent sheet and see what sort of limitations and discrepancies there might be, otherwise it might be beyond me. You are currently using the same cards and the same Diluent to carry out your manual testing as you will be using on the Instrument. Any discrepancies you see will come about because of manipulation error only. Can I suggest that you contact the local Biorad rep who installed the instruments and ask them to help you with this?
    2 points
  3. I'm sure you sent them a card.......
    1 point
  4. I agree with the sentiments above. Wiggle room is always a good idea when creating ranges for any activity/process. The art is in defining the range - certainly you don't want to be too strict that an unexpected event throws you out-of-compliance. Neither do you want ranges that are so broad that they are effectively meaningless. For maintenance, a good idea is to have a target date and then add your wiggle factor (+/- days, weeks, months, etc).
    1 point
  5. That system sounds pretty good to me. I would try to do weekly every 7 days though. If an inspector sees that most of a particular scheduled maintenance is run on the day of the week (or month) that it is supposed to be run, I do not think they will get upset about a few that is a day or two off. As far as gaps, you may want to schedule monthly maintenance somewhere in the middle of the month so it looks better if you are off a day once in a while. Scott
    1 point
×
×
  • Create New...

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.