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M antigen and antibody
MinerJ reacted to Baby Banker for a topic
Children frequently make anti-M that reacts at Coombs phase. This is usually without known sensitization. I saw this in a text book years ago, but have seen it in real life many times during my 36 years of pediatric blood banking.1 point -
Anti-M does have both IgM and IgG components. It primarily reacts as IgM but can carry over to IgG. We had one last week. The IgM component caused an ABO discrepancy by reacting with with M+ A1 reverse cells in an A Pos patient. The IgG component showed up at coombs phase after the addition of Anti-IgG. We had to transfuse M neg units to have compatible cross matches.1 point
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Yes, we use this kind of method to determine if there is ABO HDN present, putting the elution with reagent A,B,O cells respectively in the refrigerator( 4 degree C) overnight, then gently tip the tubes to see the agglutination. The O cells is as a negtive control, in case there are other cold antibodies interfere with the test.1 point
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Yes I agree Malcolm. I was thinking more of some of those reactions you get that are a bit 'iffy' in a panel in gel - which is extremely difficult to do at strictly 37 - and you just need an aid in identification1 point