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Based on your initial screen, it appears that the patient's cells are probably already coated with antibody (DAT-positive). Unless you are DTT-treating the patient's cells - not typically part of the testing protocol - they will remain DAT-positive and therefore reactive in the second series of tests using the DTT-treated screening cells.1 point
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first of all, i have not done this test before.my opinion is just from my understanding of the threats i read before, just to express myself, myabe and most likely it is not right. the auto control is the reaction of patient's cells with its own plasma, the DTT treatment is for the screening cells, if i understand it rightly. so the DTT does not do anything to the autocells.1 point
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can wash change the strength of the reaction
Malcolm Needs reacted to Yanxia for a topic
i guess the difference is because the second time's cells is more than the first time( two drops vs. one)1 point -
workup frequency for Daratumanab
Yanxia reacted to Malcolm Needs for a topic
It is, but the k antigen is almost as immunogenic as the K antigen. Given that patients that are K+k- are going to be very rare, my own thoughts are that giving them K+k- blood from the start is worthwhile, not least because, if they then do not make an anti-k, and need blood as an emergency, they can be given k+ blood to cover the emergency. This is going to be the official advice in the UK.1 point